Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging

被引：88

作者：

Bianchini, Paolo ^{[1
]}

Harke, Benjamin ^{[1
]}

Galiani, Silvia ^{[1
,2
]}

Vicidomini, Giuseppe ^{[1
]}

Diaspro, Alberto ^{[1
,2
]}

机构：

[1] Ist Italiano Tecnol, Dept Nanophys, I-16100 Genoa, Italy

[2] Univ Genoa, Dept Phys, I-16100 Genoa, Italy

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 17期

关键词：

STED MICROSCOPY; FLUORESCENCE MICROSCOPY; BRAIN-SLICES; NANOSCOPY; DEEP;

D O I：

10.1073/pnas.1119129109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

We developed a new class of two-photon excitation-stimulated emission depletion (2PE-STED) optical microscope. In this work, we show the opportunity to perform superresolved fluorescence imaging, exciting and stimulating the emission of a fluorophore by means of a single wavelength. We show that a widely used red-emitting fluorophore, ATTO647N, can be two-photon excited at a wavelength allowing both 2PE and STED using the very same laser source. This fact opens the possibility to perform 2PE microscopy at four to five times STED-improved resolution, while exploiting the intrinsic advantages of nonlinear excitation.

引用

页码：6390 / 6393

页数：4

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