Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat

被引：39

作者：

Fukui, KN

Suzuki, G

Lagudah, ES

Rahman, S

Appels, R

Yamamoto, M

Mukai, Y ^{[1
]}

机构：

[1] Osaka Kyoiku Univ, Div Nat Sci, Kashihara, Nara 5828582, Japan

[2] Commonwealth Sci & Ind Res Org, Canberra, ACT 2601, Australia

[3] Kansai Womens Coll, Kashiwara 5820026, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2001年 / 42卷 / 02期

关键词：

centromere; fluorescence in situ hybridization; (FISH); large-insert library; retrotransposon; wheat;

D O I：

10.1093/pcp/pce026

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cereal centromeres commonly contain many repetitive sequences that are derived from Ty3/gypsy retrotransposon, FISH analysis using a large DNA insert Library of wheat identified a 67-kb clone (R11H) that showed strong hybridization signals on the centromeres. The R11H clone contains Ty3/gypsy retrotransposon-related sequences; both integrase and CCS1 family sequences were identified, Subsequently, we isolated additional 23 large-insert clones which also contained the integrase and CCS1 sequences. Based on the number of the integrase repeats in the clones determined by DNA gel blot analysis, we concluded that the retrotransposon-like sequences are tandemly repeated in wheat centromeres in ca, 55-kb interval on average, This conclusion is consistent with the results of FISH analysis on the extended DNA fibers.

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收藏

页码：189 / 196

页数：8

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