A novel osteopontin-like protein is expressed in the trout ovary during ovulation

被引：19

作者：

Bobe, J

Goetz, FW

机构：

[1] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

[2] SCRIBE, Inst Nacl Rech Agron, F-35042 Rennes, France

来源：

FEBS LETTERS | 2001年 / 489卷 / 2-3期

关键词：

ovulation; trout; ovary; osteopontin; suppression subtraction hybridization;

D O I：

10.1016/S0014-5793(01)02090-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using suppression subtraction hybridization between ovulatory and postovulatory trout ovaries, a down-regulated cDNA was obtained that presumably encodes a novel ovarian protein ('NOP'), NOP mRNA is present in the ovary during ovulation and down-regulated by 48 h postovulation, suggesting an important role for NOP during ovulation, Besides the ovary, NOP is also strongly expressed in the testis and at lower levels in the skin, gills, kidney and gastrointestinal tract. While the overall identity is not high, NOP shares several sequence similarities with mammalian and chicken osteopontins, including the percentage of aspartate, serine and alanine residues and the presence of a cell attachment motif, (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：119 / 124

页数：6

共 31 条

[1] INVITRO OVULATION, PROSTAGLANDIN SYNTHESIS, AND PROTEOLYSIS IN ISOLATED OVARIAN COMPONENTS OF YELLOW PERCH (PERCA-FLAVESCENS) - EFFECTS OF 17-ALPHA,20-BETA-DIHYDROXY-4-PREGNEN-3-ONE AND PHORBOL ESTER [J].