Enhanced production of scopolamine by bacterial elicitors in adventitious hairy root cultures of Scopolia parviflora

被引:36
作者
Jung, HY
Kang, SM
Kang, YM
Kang, MJ
Yun, DJ
Bahk, JD
Yang, JK
Choi, MS [1 ]
机构
[1] Gyeongsang Natl Univ, Dept Forest Sci, Jinju, South Korea
[2] Gyeongsang Natl Univ, Div Plant Mol Biol, Jinju, South Korea
关键词
adventitious hairy root; bacterial elicitors; Scopolia parviflora; tropane alkaloids; H6H; PMT;
D O I
10.1016/S0141-0229(03)00253-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In an attempt to increase productivity, the effect of elicitation on tropane alkaloids (TA) biosynthesis was studied in adventitious hairy root cultures of Scopolia parviflora. Two Gram-positive strains and one Gram-negative strain of bacteria were used as biotic elicitors. The raw bacterial elicitors affected the tropane alkaloid profile by increasing the scopolamine concentration, while the autoclaved bacterial elicitors produced similar effects on the control. The conversion ratio of hyoscyamine to scopolamine was increased following elicitation using raw bacterial elicitors. The bacterial elicitor inhibited the expression of H6H (hyoscyamine 6beta-hydoxylase) whereas the expression of PMT (putrescine N-methyltransferase) was raised by elicitation. These results have important implications for the large-scale production of tropane alkaloids. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:987 / 990
页数:4
相关论文
共 8 条
[1]   Influence of feeding precursors on tropane alkaloid production during an abiotic stress in Datura innoxia transformed roots [J].
Boitel-Conti, M ;
Laberche, JC ;
Lanoue, A ;
Ducrocq, C ;
Sangwan-Norreel, BS .
PLANT CELL TISSUE AND ORGAN CULTURE, 2000, 60 (02) :131-137
[2]   ELICITING SECONDARY METABOLISM IN PLANT-CELL CULTURES [J].
DICOSMO, F ;
MISAWA, M .
TRENDS IN BIOTECHNOLOGY, 1985, 3 (12) :318-322
[3]  
EBEL J, 1986, ANNU REV PHYTOPATHOL, V24, P235
[4]   Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering [J].
Jouhikainen, K ;
Lindgren, L ;
Jokelainen, T ;
Hiltunen, R ;
Teeri, TH ;
Oksman-Caldentey, KM .
PLANTA, 1999, 208 (04) :545-551
[5]  
Jung H, 2002, KOREAN J BIOTECHNOL, V7, P525
[6]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[7]   CHALCONE ISOMERASE CDNA CLONING AND MESSENGER-RNA INDUCTION BY FUNGAL ELICITOR, WOUNDING AND INFECTION [J].
MEHDY, MC ;
LAMB, CJ .
EMBO JOURNAL, 1987, 6 (06) :1527-1533
[8]  
Oksman-Caldentey KM, 2000, METABOLIC ENG PLANT, P253, DOI [DOI 10.1007/978-94-015-9423-3_13, 10.1007/978-94-015-9423-3_13]