Separation of radioactive metabolites in cultured tea cells fed with [14C]phenylalanine using high-speed counter-current chromatography

被引：7

作者：

Du, QZ

Cheng, H

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Hangzhou Univ Commerce, Inst Food & Biol Engn, Hangzhou 310035, Peoples R China

[3] Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 31008, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 921卷 / 02期

关键词：

counter-current chromatography; tea; catechins; polyphenols;

D O I：

10.1016/S0021-9673(01)00862-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Separation of radioactive metabolites in cultured tea cells fed with [C-14]phenylalanine was conducted using high-speed counter-current chromatography. Among seven components obtained our studies focused two metabolites, i.e. (-)-epicatechin and D,L-catechin. The specific radioactivity of (-)-epicatechin was 212.01 KBq/mg, amounting to 8.5% of the total radioactivity of ethyl acetate extract while that of D,L-catechin was 1.0006 MBq/mg or 5.4% of the total. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：331 / 334

页数：4

共 7 条

[1]

BOONE CW, 1990, CANCER RES, V50, P2

[2]

CHENG H, 2000, THESIS ZHEIJING U HA

[3] PURIFICATION OF (-)-EPIGALLOCATECHIN FROM ENZYMATIC HYDROLYSATE OF ITS GALLATE USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY [J].