Evaluation of Short and Long Term Cold Stress Challenge of Nerve Grow Factor, Brain-Derived Neurotrophic Factor, Osteocalcin and Oxytocin mRNA Expression in BAT, Brain, Bone and Reproductive Tissue of Male Mice Using Real-Time PCR and Linear Correlation Analysis

被引:22
作者
Camerino, Claudia [1 ,2 ]
Conte, Elena [2 ]
Caloiero, Roberta [2 ]
Fonzino, Adriano [2 ]
Carratu, Mariarosaria [1 ]
Lograno, Marcello D. [2 ]
Tricarico, Domenico [2 ]
机构
[1] Univ Bari Aldo Moro, Dept Basic Med Sci Neurosci & Sense Organs, Bari, Italy
[2] Univ Bari Aldo Moro, Dept Pharm Drug Sci, Bari, Italy
关键词
osteocalcin; nerve growth factor; brain-derived neurotrophic factor; oxytocin; gene expression; cold stress; ENERGY-METABOLISM; RESTRAINT STRESS; SKELETAL-MUSCLE; ADIPOSE-TISSUE; OSTEOBLASTS; CHANNELS; INSULIN; INVOLVEMENT; PHENOTYPE; DELETION;
D O I
10.3389/fphys.2017.01101
中图分类号
Q4 [生理学];
学科分类号
071003 [生理学];
摘要
The correlation between the Ngf/p75ntr-Ntrk1 and Bdnf, Osteocalcin-Ost/Gprc6a and Oxytocin-Oxt/Oxtr genes, was challenged investigating their mRNA levels in 3 months-old mice after cold-stress (CS). Uncoupling protein-1 (Ucp-1) was used as positive control. Control mice were maintained at room temperature T = 25 degrees C, CS mice were maintained at T = 4 degrees C for 6 h and 5-days (N = 15 mice). RT-PCR experiments showed that Ucp-1 and Ngf genes were up-regulated after 6 h CS in brown adipose tissues (BAT), respectively, by 2 and 1.5-folds; Ucp-1 was upregulated also after 5-days, while Ngfr (p75ntr) and Ntrk1 genes were downregulated after 6 h and 5-days CS in BAT. NGF and P75NTR were upregulated in bone and testis following 5-days, and P75NTR in testis after 6 h CS. Bdnf was instead up-regulated in bone following 5-days CS and down-regulated in testis. OST was upregulated by 16 and 3-fold in bone and BAT, respectively, following 5-days CS. Gprc6a was upregulated after 6 h in brain, while Bglap (Ost) gene was downregulated. Oxt gene was upregulated by 5-fold following 5-days CS in bone. Oxtr was upregulated by 0.5 and 0.3-fold, respectively, following 6 h and 5-days CS in brain. Oxtr and Oxt were downregulated in testis and in BAT. The changes in the expression levels of control genes vs. genes following 6 h and 5-days CS were correlated in all tissues, but not in BAT. Correlation in BAT was improved eliminating Ngfr (p75ntr) data. The correlation in brain was lost eliminating Oxtr data. In sum, Ucp-1 potentiation in BAT after cold stress is associated with early Ngf-response in the same tissue and trophic action in bone and testis. In contrast, BDNF exerts bone and neuroprotective effects. Similarly to Ucp-1, Bglap (Ost) signaling is enhanced in bone and BAT while it may exert local neuroprotective effects thought its receptor. Ngfr (p75ntr) regulates the adaptation to CS through a feed-back loop in BAT. Oxtr regulates the gene-response to CS through a feed-forward loop in brain. Overall these results expand the understanding of the physiology of these molecules under metabolic thermogenesis.
引用
收藏
页数:8
相关论文
共 38 条
[1]
Short-term moderate hypothermia stimulates alkaline phosphatase activity and osteocalcin expression in osteoblasts by upregulating Runx2 and osterix in vitro [J].
Aisha, M. D. ;
Nor-Ashikin, M. N. K. ;
Sharaniza, A. B. ;
Nawawi, H. M. ;
Kapitonova, M. Y. ;
Froemming, G. R. A. .
EXPERIMENTAL CELL RESEARCH, 2014, 326 (01) :46-56
[2]
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].
Bustin, Stephen A. ;
Benes, Vladimir ;
Garson, Jeremy A. ;
Hellemans, Jan ;
Huggett, Jim ;
Kubista, Mikael ;
Mueller, Reinhold ;
Nolan, Tania ;
Pfaffl, Michael W. ;
Shipley, Gregory L. ;
Vandesompele, Jo ;
Wittwer, Carl T. .
CLINICAL CHEMISTRY, 2009, 55 (04) :611-622
[3]
Central Depletion of Brain-Derived Neurotrophic Factor in Mice Results in High Bone Mass and Metabolic Phenotype [J].
Camerino, C. ;
Zayzafoon, M. ;
Rymaszewski, M. ;
Heiny, J. ;
Rios, M. ;
Hauschka, P. V. .
ENDOCRINOLOGY, 2012, 153 (11) :5394-5405
[4]
Camerino C., 2009, IBMS BONEKEY, V6, P295, DOI [10.1138/20090392), DOI 10.1138/20090392, 10.1138/20090392]
[5]
Camerino C., 2012, IBMS BONEKEY, V202, P1, DOI [10.1038/bonekey.2012.202, DOI 10.1038/BONEKEY.2012.202]
[6]
Nerve Growth Factor, Brain-Derived Neurotrophic Factor and Osteocalcin Gene Relationship in Energy Regulation, Bone Homeostasis and Reproductive Organs Analyzed by mRNA Quantitative Evaluation and Linear Correlation Analysis [J].
Camerino, Claudia ;
Conte, Elena ;
Cannone, Maria ;
Caloieros, Roberta ;
Fonzino, Adriano ;
Tricarico, Domenico .
FRONTIERS IN PHYSIOLOGY, 2016, 7
[7]
Low Sympathetic Tone and Obese Phenotype in Oxytocin-deficient Mice [J].
Camerino, Claudia .
OBESITY, 2009, 17 (05) :980-984
[8]
Effects of the Antidiabetic Drugs on the Age-Related Atrophy and Sarcopenia Associated with Diabetes Type II [J].
Cetrone, Michela ;
Mele, Antonietta ;
Tricarico, Domenico .
CURRENT DIABETES REVIEWS, 2014, 10 (04) :231-237
[9]
Kinetics of Oxytocin Response to Repeated Restraint Stress and/or Chronic Cold Exposure [J].
Danevova, V. ;
Kvetnansky, R. ;
Jezova, D. .
HORMONE AND METABOLIC RESEARCH, 2013, 45 (12) :845-848
[10]
Oxytocin is an age-specific circulating hormone that is necessary for muscle maintenance and regeneration [J].
Elabd, Christian ;
Cousin, Wendy ;
Upadhyayula, Pavan ;
Chen, Robert Y. ;
Chooljian, Marc S. ;
Li, Ju ;
Kung, Sunny ;
Jiang, Kevin P. ;
Conboy, Irina M. .
NATURE COMMUNICATIONS, 2014, 5