Development of a competitive index assay to evaluate the virulence of Listeria monocytogenes actA mutants during primary and secondary infection of mice

被引：67

作者：

Auerbuch, V

Lenz, LL

Portnoy, DA

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 09期

关键词：

D O I：

10.1128/IAI.69.9.5953-5957.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We developed a competitive index assay for murine listeriosis that tests the virulence of Listeria monocytogenes strains in different organs and at various times postinoculation. Studies presented here demonstrate the reproducibility of this assay during primary and secondary infection of inbred and outbred mice. We verified the validity of this assay by performing competitive index analysis of a well-characterized strain of L. monocytogenes lacking the actA gene. In addition, we found that while L. monocytogenes strains unable to recruit vasodilator-stimulated phosphoprotein (VASP) to their surface exhibit a 10-fold virulence attenuation in the livers of naive animals, they display a 50-fold survival defect in the liver during secondary listeriosis.

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页码：5953 / 5957

页数：5

相关论文

共 28 条

[1] Salmonella maintains the integrity of its intracellular vacuole through the action of SifA [J].

Beuzón, CR ;

Méresse, S ;

Unsworth, KE ;

Ruíz-Albert, J ;

Garvis, S ;

Waterman, SR ;

Ryder, TA ;

Boucrot, E ;

Holden, DW .

EMBO JOURNAL, 2000, 19 (13) :3235-3249

[2]

BISHOP DK, 1987, J IMMUNOL, V139, P2005

[3] EXPRESSION AND PHOSPHORYLATION OF THE LISTERIA-MONOCYTOGENES ACTA PROTEIN IN MAMMALIAN-CELLS [J].

BRUNDAGE, RA ;

SMITH, GA ;

CAMILLI, A ;

THERIOT, JA ;

PORTNOY, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :11890-11894

[4] Coordinate regulation of complex T cell populations responding to bacterial infection [J].

Busch, DH ;

Pilip, IM ;

Vijh, S ;

Pamer, EG .

IMMUNITY, 1998, 8 (03) :353-362

[5] DUAL ROLES OF PLCA IN LISTERIA-MONOCYTOGENES PATHOGENESIS [J].

CAMILLI, A ;

TILNEY, LG ;

PORTNOY, DA .

MOLECULAR MICROBIOLOGY, 1993, 8 (01) :143-157

[6] INSERTIONAL MUTAGENESIS OF LISTERIA-MONOCYTOGENES WITH A NOVEL TN917 DERIVATIVE THAT ALLOWS DIRECT CLONING OF DNA FLANKING TRANSPOSON INSERTIONS [J].

CAMILLI, A ;

PORTNOY, DA ;

YOUNGMAN, P .

JOURNAL OF BACTERIOLOGY, 1990, 172 (07) :3738-3744

[7] NEUTROPHILS ARE ESSENTIAL FOR EARLY ANTI-LISTERIA DEFENSE IN THE LIVER, BUT NOT IN THE SPLEEN OR PERITONEAL-CAVITY, AS REVEALED BY A GRANULOCYTE-DEPLETING MONOCLONAL-ANTIBODY [J].

CONLAN, JW ;

NORTH, RJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (01) :259-268

[8] A NOVEL BACTERIAL VIRULENCE GENE IN LISTERIA-MONOCYTOGENES REQUIRED FOR HOST-CELL MICROFILAMENT INTERACTION WITH HOMOLOGY TO THE PROLINE-RICH REGION OF VINCULIN [J].

DOMANN, E ;

WEHLAND, J ;

ROHDE, M ;

PISTOR, S ;

HARTL, M ;

GOEBEL, W ;

LEIMEISTERWACHTER, M ;

WUENSCHER, M ;

CHAKRABORTY, T .

EMBO JOURNAL, 1992, 11 (05) :1981-1990

[9] LISTERIA-MONOCYTOGENES, A FOOD-BORNE PATHOGEN [J].

FARBER, JM ;

PETERKIN, PI .

MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :476-511

[10]

FARBER JM, 1991, MICROBIOL REV, V55, P752