A strain of Pseudomonas stutzeri coded 1317 isolated from oil-contaminated soil was found to grow well in glucose and soybean oil as a sore carbon source, respectively. Polyhydroxyalkanoates containing medium chain length monomers of hydroxyalkanoates ranging from C6 to C14 were synthesized up to 52% of cell dry weight of P. stutzri 1317 grown on glucose mineral media. In mineral media containing 10 g l(-1) soybean oil, P. stutzert 1317 produced up to 63% of polyhydroxyalkanoates containing mainly a novel monomer of 3,6-epoxy-7-nonene-1,9 dioic acids making up to 63% of the polyhydroxyalkanoates, together with minor monomers of C8 and C10. H-1- and C-13-nuclear magnetic resonance spectroscopy and electron impact gas chromatography-mass spectroscopy and chemical ionization gas chromatography-mass spectroscopy were used to study the structures of polyhydroxyalkanoates synthesized by P. stutzeri 1317. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
机构:
GYEONGSANG NATL UNIV, DEPT MICROBIOL, BIOMACROMOLEC RES LAB, CHINJU 660701, SOUTH KOREAGYEONGSANG NATL UNIV, DEPT MICROBIOL, BIOMACROMOLEC RES LAB, CHINJU 660701, SOUTH KOREA
机构:
GYEONGSANG NATL UNIV, DEPT MICROBIOL, BIOMACROMOLEC RES LAB, CHINJU 660701, SOUTH KOREAGYEONGSANG NATL UNIV, DEPT MICROBIOL, BIOMACROMOLEC RES LAB, CHINJU 660701, SOUTH KOREA