A map of the interactome network of the metazoan C-elegans

被引：1253

作者：

Li, SM

Armstrong, CM

Bertin, N

Ge, H

Milstein, S

Boxem, M

Vidalain, PO

Han, JDJ

Chesneau, A

Hao, T

Goldberg, DS

Li, N

Martinez, M

Rual, JF

Lamesch, P

Xu, L

Tewari, M

Wong, SL

Zhang, LV

Berriz, GF

Jacotot, L

Vaglio, P

Reboul, J

Hirozane-Kishikawa, T

Li, QR

Gabel, HW

Elewa, A

Baumgartner, B

Rose, DJ

Yu, HY

Bosak, S

Sequerra, R

Fraser, A

Mango, SE

Saxton, WM

Strome, S

van den Heuvel, S

Piano, F

Vandenhaute, J

Sardet, C

Gerstein, M

Doucette-Stamm, L

Gunsalus, KC

Harper, JW

Cusick, ME

Roth, FP

Hill, DE

Vidal, M

机构：

[1] Dana Farber Canc Inst, Ctr Canc Syst Biol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[3] Inst Genet Mol, CNRS, UMR 5535, F-34293 Montpellier 5, France

[4] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[5] Fac Notre Dame Paix, Unite Rech Biol Mol, B-5000 Namur, Belgium

[6] Baylor Coll Med, Verna & Marrs Dept Biochem & Mol Biol, Program Cell & Mol Biol Biophys & Biol, Houston, TX 77030 USA

[7] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

[8] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[9] Yale Univ, Dept Comp Sci, New Haven, CT 06520 USA

[10] Agencourt Biosci Corp, Beverly, MA 01915 USA

[11] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[12] Univ Utah, Huntsman Canc Inst, Salt Lake City, UT 84112 USA

[13] Massachusetts Gen Hosp, Ctr Canc, Charlestown, MA 02129 USA

[14] NYU, Dept Biol, New York, NY 10003 USA

来源：

SCIENCE | 2004年 / 303卷 / 5657期

关键词：

D O I：

10.1126/science.1091403

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains similar to5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

引用

页码：540 / 543

页数：4

共 27 条

[1] Combined functional genomic maps of the C-elegans DNA damage response [J].

Boulton, SJ ;

Gartner, A ;

Reboul, J ;

Vaglio, P ;

Dyson, N ;

Hill, DE ;

Vidal, M .

SCIENCE, 2002, 295 (5552) :127-131

[2]

Ch'ng Q, 1999, DEVELOPMENT, V126, P3303

[3] YPD™, PombePD™ and WormPD™:: model organism volumes of the BioKnowledge™ Library, an integrated resource for protein information [J].

Costanzo, MC ;

Crawford, ME ;

Hirschman, JE ;

Kranz, JE ;

Olsen, P ;

Robertson, LS ;

Skrzypek, MS ;

Braun, BR ;

Hopkins, KL ;

Kondu, P ;

Lengieza, C ;

Lew-Smith, JE ;

Tillberg, M ;

Garrels, JI .

NUCLEIC ACIDS RESEARCH, 2001, 29 (01) :75-79

[4] A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome [J].

Davy, A ;

Bello, P ;

Thierry-Mieg, N ;

Vaglio, P ;

Hitti, J ;

Doucette-Stamm, L ;

Thierry-Mieg, D ;

Reboul, J ;

Boulton, S ;

Walhout, AJM ;

Coux, O ;

Vidal, M .

EMBO REPORTS, 2001, 2 (09) :821-828

[5] The Caenorhabditis elegans Six/sine oculis class homeobox gene ceh-32 is required for head morphogenesis [J].

Dozier, C ;

Kagoshima, H ;

Niklaus, G ;

Cassata, G ;

Bürglin, TR .

DEVELOPMENTAL BIOLOGY, 2001, 236 (02) :289-303

[6] Regulation of organogenesis by the Caenorhabditis elegans, FoxA protein PHA-41 [J].

Gaudet, J ;

Mango, SE .

SCIENCE, 2002, 295 (5556) :821-825

[7] Functional organization of the yeast proteome by systematic analysis of protein complexes [J].

Gavin, AC ;

Bösche, M ;

Krause, R ;

Grandi, P ;

Marzioch, M ;

Bauer, A ;

Schultz, J ;

Rick, JM ;

Michon, AM ;

Cruciat, CM ;

Remor, M ;

Höfert, C ;

Schelder, M ;

Brajenovic, M ;

Ruffner, H ;

Merino, A ;

Klein, K ;

Hudak, M ;

Dickson, D ;

Rudi, T ;

Gnau, V ;

Bauch, A ;

Bastuck, S ;

Huhse, B ;

Leutwein, C ;

Heurtier, MA ;

Copley, RR ;

Edelmann, A ;

Querfurth, E ;

Rybin, V ;

Drewes, G ;

Raida, M ;

Bouwmeester, T ;

Bork, P ;

Seraphin, B ;

Kuster, B ;

Neubauer, G ;

Superti-Furga, G .

NATURE, 2002, 415 (6868) :141-147

[8] Integrating 'omic' information: a bridge between genomics and systems biology [J].

Ge, H ;

Walhout, AJM ;

Vidal, M .

TRENDS IN GENETICS, 2003, 19 (10) :551-560

[9]

GE H, UNPUB

[10] Assessing experimentally derived interactions in a small world [J].

Goldberg, DS ;

Roth, FP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (08) :4372-4376

← 1 2 3 →