GM-CSF-deficient mice are susceptible to pulmonary group B Streptococcal infection

Granulocyte-macrophage colony-stimulating factor (GM-CSF) gene-targeted mice (GM(-/-)) cleared group B streptococcus (GBS) from the lungs more slowly than wild-type mice. Expression of GM-CSF in the respiratory epithelium of GM(-/-) mice improved bacterial clearance to levels greater than that in wildtype GM(+/+) mice. Acute aerosolization of GM-CSF to GM(+/+) mice significantly enhanced clearance of GBS at 24 hours. GBS infection was associated with increased neutrophilic infiltration in lungs of GM(-/-) mice, while macrophage infiltrates predominated in wild-type mice, suggesting an abnormality in macrophage clearance of bacteria in the absence of GM-CSF. While phagocytosis of GBS was unaltered, production of superoxide radicals and hydrogen peroxide was markedly deficient in macrophages from GM(-/-) mice. Lipid peroxidation, assessed by measuring the isoprostane 8-iso-PGF(2 alpha), was decreased in the lungs of GM(-/-) mice. GM-CSF plays an important role in GBS clearance itt vivo, mediated in part by its role in enhancing superoxide and hydrogen peroxide production and bacterial killing by alveolar macrophages.