An improved procedure for the isolation of nuclear DNA from leaves of wild grapevine dried with silica gel

被引:43
作者
Wang, XD [1 ]
Wang, ZP [1 ]
Zou, YP [1 ]
机构
[1] CHINESE ACAD SCI,INST BOT,LAB SYSTEMAT & EVOLUTIONARY BOT,BEIJING 100093,PEOPLES R CHINA
关键词
isolation; nuclear DNA; RAPD; silica gel; Vitis;
D O I
10.1007/BF02673369
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An efficient and convenient method is presented for the isolation of nuclear DNA from leaves of wild Vitis species that have been dried with silica gel. The nuclear DNA obtained with this method is suitable for both PCR amplification and digestion with restriction endonucleases.
引用
收藏
页码:369 / 373
页数:5
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