Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length

被引：178

作者：

Miyoshi, Tomoichiro ^{[1
]}

Kanoh, Junko ^{[1
]}

Saito, Motoki ^{[1
]}

Ishikawa, Fuyuki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Dept Gene Mech, Sakyo Ku, Kyoto 6068501, Japan

来源：

SCIENCE | 2008年 / 320卷 / 5881期

关键词：

D O I：

10.1126/science.1154819

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Telomeres are specialized chromatin structures that protect chromosomal ends. Protection of telomeres 1 ( Pot1) binds to the telomeric G- rich overhang, thereby protecting telomeres and regulating telomerase. Mammalian POT1 and TPP1 interact and constitute part of the six- protein shelterin complex. Here we report that Tpz1, the TPP1 homolog in fission yeast, forms a complex with Pot1. Tpz1 binds to Ccq1 and the previously undiscovered protein Poz1 ( Pot1- associated in Schizosaccharomyces pombe), which protect telomeres redundantly and regulate telomerase in positive and negative manners, respectively. Thus, the Pot1- Tpz1 complex accomplishes its functions by recruiting effector molecules Ccq1 and Poz1. Moreover, Poz1 bridges Pot1- Tpz1 and Taz1- Rap1, thereby connecting the single- stranded and double- stranded telomeric DNA regions. Such molecular architectures are similar to those of mammalian shelterin, indicating that the overall DNA- protein architecture is conserved across evolution.

引用

页码：1341 / 1344

页数：4

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