Effect of the HP0159ORF mutation on the lipopolysaccharide structure and colonizing ability of Helicobacter pylori

被引：17

作者：

Altman, Eleonora ^{[1
]}

Chandan, Vandana ^{[1
]}

Larocque, Suzon ^{[1
]}

Aubry, Annie ^{[1
]}

Logan, Susan M. ^{[1
]}

Vinogradov, Evgeny ^{[1
]}

Li, Jianjun ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2008年 / 53卷 / 02期

关键词：

Helicobacter pylori; lipopolysaccharide; structure; colonization;

D O I：

10.1111/j.1574-695X.2008.00416.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

The outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an alpha-1,6-glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal DD-heptose residue in the side-chain outer core fragment and maintaining an inner core backbone structure compared with the wild-type Lewis antigen-expressing strains. Colonization studies with HP0159 mutants of two mouse-colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach.

引用

页码：204 / 213

页数：10

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