The HUman MicroNucleus project on eXfoLiated buccal cells (HUMNXL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

被引：294

作者：

Bonassi, Stefano ^{[1
]}

Coskun, Erdem ^{[1
,2
]}

Ceppi, Marcello ^{[3
]}

Lando, Cecilia ^{[3
]}

Bolognesi, Claudia ^{[4
]}

Burgaz, Sema ^{[5
]}

Holland, Nina ^{[5
]}

Kirsh-Volders, Micheline ^{[6
]}

Knasmueller, Siegfried ^{[7
]}

Zeiger, Errol ^{[8
]}

Carnesoltas, Deyanira ^{[9
]}

Cavallo, Delia ^{[10
]}

da Silva, Juliana ^{[11
]}

de Andrade, Vanessa M. ^{[12
]}

Demircigil, Gonca Cakmak ^{[2
]}

Dominguez Odio, Anibal ^{[13
]}

Donmez-Altuntas, Hamiyet ^{[14
]}

Gattas, Gilka ^{[15
]}

Giri, Ashok ^{[16
]}

Giri, Sarbani ^{[17
]}

Gomez-Meda, Belinda ^{[18
]}

Gomez-Arroyo, Sandra ^{[19
]}

Hadjidekova, Valeria ^{[20
]}

Haveric, Anja ^{[21
]}

Kamboj, Mala ^{[22
]}

Kurteshi, Kemajl ^{[23
]}

Martino-Roth, Maria Grazia ^{[24
]}

Montero Montoya, Regina ^{[25
]}

Nersesyan, Armen ^{[7
,26
]}

Pastor-Benito, Susana ^{[27
]}

Favero Salvadori, Daisy Maria ^{[28
]}

Shaposhnikova, Alina ^{[29
]}

Stopper, Helga ^{[30
]}

Thomas, Philip ^{[31
]}

Torres-Bugarin, Olivia ^{[32
]}

Yadav, Abhay Singh ^{[33
]}

Zuniga Gonzalez, Guillermo ^{[34
]}

Fenech, Michael ^{[31
]}

机构：

[1] IRCCS San Raffaele Pisana, Unit Clin & Mol Epidemiol, I-00166 Rome, Italy

[2] Gazi Univ, Dept Toxicol, Fac Pharm, TR-06330 Ankara, Turkey

[3] Natl Inst Canc Res, Unit Environm Epidemiol, Genoa, Italy

[4] Natl Inst Canc Res, Unit Environm Carcinogenesis, Genoa, Italy

[5] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA

[6] Vrije Univ Brussel, Lab Cell Genet, Brussels, Belgium

[7] Med Univ, Inst Canc Res, Vienna, Austria

[8] Errol Zeiger Consulting, Chapel Hill, NC USA

[9] Natl Inst Oncol & Radiobiol, Lab Clin & Expt Pharmacol, Havana 10400, Cuba

[10] INAIL, Dept Occupat Med, I-00040 Rome, Italy

[11] Univ Luterana Brasil ULBRA, Lab Geneet Toxicol, Curso Biol, Canoas, RS, Brazil

[12] Univ Extremo Catarinense, Lab Cellular & Mol Biol, Programa Posgrad Ciencias Saude, Criciuma, SC, Brazil

[13] Lab Prod Biofarmaceut & Quim LABIOFAM, Direcc Invest & Desarrollo, Havana, Cuba

[14] Erciyes Univ, Med Biol Lab, Fac Med, TR-38039 Kayseri, Turkey

[15] Univ Sao Paulo, Lab Citogenet & Biol Mol, Dept Med Legal Erica Med & Med Social & Trabalho, Fac Med, BR-09500900 Sao Paulo, Brazil

[16] Indian Inst Chem Biol, Mol & Human Genet Div, Div Human Genet & Genom, Kolkata, India

[17] Assam Univ, Lab Genet Toxicol, Dept Life Sci & Bioinformat, Silchar, Assam, India

[18] Univ Guadalajara, Inst Biol Mol Med & Terapia Genica, Ctr Univ Ciencias Salud, Guadalajara 44340, Jalisco, Mexico

[19] Univ Nacl Autonoma Mexico, Lab Citogenet Ambiental, Ctr Ciencias Atmosfera, Mexico City 04510, DF, Mexico

[20] Natl Ctr Radiobiol & Radiat Protect, Lab Radiat Genet, Sofia 1606, Bulgaria

[21] Univ Sarajevo, Lab Cytogenet & Genotoxicol, Inst Genet Engn & Biotechnol, Sarajevo 71000, Bosnia & Herceg

[22] Career Post Grad Inst Dent Sci & Hosp, Dept Oral Pathol & Microbiol, Lucknow 226003, Uttar Pradesh, India

[23] Univ Prishtina, Genet Lab, Dept Biol, Fac Nat Sci, Prishtina, Kosova, Serbia

[24] Univ Fed Pelotas, Dept Zool & Genet, Escola Saude, Pelotas, Brazil

[25] Univ Nacl Autonoma Mexico, Dpto Med Genom & Toxicol Ambiental, Inst Invest Biomed, Mexico City 70228, DF, Mexico

[26] Natl Oncol Ctr, Yerevan 0052, Armenia

[27] Univ Autonoma Barcelona, Dept Genet & Microbiol, Fac Biociencies, Bellaterra 08193, Spain

[28] UNESP, Lab Toxicogenom & Nutrigenom, Dept Patol, Fac Med Botucatu,Univ Estadual Paulista, Botucatu, SP, Brazil

[29] Kemerovo State Univ, Genet Lab, Kemerovo 650043, Russia

[30] Univ Wurzburg, Dept Toxicol, D-97078 Wurzburg, Germany

[31] CSIRO Food & Nutr Sci, Adelaide, SA 5000, Australia

[32] Univ Autonoma Guadalajara, Lab Invest, Programa Int, Fac Med, Guadalajara, Jalisco, Mexico

[33] Kurukshetra Univ, Human Genet Lab, Dept Zool, Kurukshetra 136119, Haryana, India

[34] Inst Mexicano Seguro Social, Lab Mutagenesis, Ctr Invest Biomed Occidente, Guadalajara, Jalisco, Mexico

来源：

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH | 2011年 / 728卷 / 03期

关键词：

Buccal micronucleus cytome assay (BMCyt); Genetic instability; Exfoliated cell; Pooled analysis; Molecular epidemiology; Assay standardization; PERIPHERAL-BLOOD LYMPHOCYTES; GENETIC POLYMORPHISMS; DNA-DAMAGE; MUCOSA CELLS; ORAL-MUCOSA; FREQUENCY; CYTOME; SMOKING; DISEASE;

D O I：

10.1016/j.mrrev.2011.06.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMNXL, collaborative study. The HUMNXL, project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74 parts per thousand (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (p < 0.001). Most occupational exposures and a diagnosis of cancer significantly increased MN and other endpoints frequencies. MN frequency increased in heavy smoking (>= 40 cig/day. FR = 1.37:95% CI 1.03-.82) and decreased with daily fruit consumption (FR = 0.68; 95% CI 0.50-0.91). The results of the HUMNXL, project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：88 / 97

页数：10

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