Group IB phospholipase A2 from Pseudonaja textilis

被引:17
作者
Armugam, A [1 ]
Gong, NL [1 ]
Li, XJ [1 ]
Siew, PY [1 ]
Chai, SC [1 ]
Nair, R [1 ]
Jeyaseelan, K [1 ]
机构
[1] Natl Univ Singapore, Fac Med, Dept Biochem, Singapore 117597, Singapore
关键词
phospholipase A(2) gene; common brown snake; procoagulant and Pseudonaja;
D O I
10.1016/j.abb.2003.09.045
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they showed procoagulant property. cDNA cloning and characterization indicated the presence of two isoforms of PLA(2) proteins in a single snake, each containing the "pancreatic loop," characteristic of group IB phospholipase A(2). The genomic cloning also confirmed the presence of two genes each containing four exons that are interrupted by three introns. Phylogenetic analysis showed that the venom group IB PLA(2) gene is primitive and could have evolved from the same ancestor as the mammalian and venom group IA PLA(2) genes. In the present study, we report that the Pt-PLA2 gene could be responsible for the production of PL1, 2, and 3 possibly via RNA editing process. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:10 / 20
页数:11
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