Determination of LSD in urine with high-performance liquid chromatography-mass spectrometry

被引：5

作者：

Bodin, K ^{[1
]}

Svensson, JO ^{[1
]}

机构：

[1] Huddinge Univ Hosp, Dept Clin Pharmacol, S-14186 Huddinge, Sweden

来源：

THERAPEUTIC DRUG MONITORING | 2001年 / 23卷 / 04期

关键词：

analysis; atmospheric pressure ionization; electrospray; high-performance liquid chromatography; LSD; mass spectrometry; urine;

D O I：

10.1097/00007691-200108000-00011

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A rapid, specific, and sensitive high-performance liquid chromatography/mass spectrometry method has been developed for routine determination of lysergic acid diethylamide (LSD) in urine. It includes sample purification by extraction into an organic solvent and back-extraction to an acetate buffer, reversed-phase highperformance liquid chromatography, and detection with a single quadrupole mass spectrometer equipped with an atmospheric pressure ionization electrospray interface. Trideuterated LSD was used as internal standard. The limit of detection was 0.02 ng/mL and the calibration curve was linear from 0.05 to 10 ng/mL. Within- and between-day coefficients of variation were 3.5% and 4.0% respectively and extraction recovery was 91%.

引用

页码：389 / 393

页数：5

共 10 条

[1] CHROMATOGRAPHIC METHOD FOR DETECTION OF LSD IN BIOLOGICAL LIQUIDS [J].

CHRISTIE, J ;

WHITE, MW ;

WILES, JM .

JOURNAL OF CHROMATOGRAPHY, 1976, 120 (02) :496-501

[2]

FRANCOM P, 1988, J ANAL TOXICOL, V12, P1

[3] Determination of LSD and N-demethyl-LSD in urine by liquid chromatography coupled to electrospray ionization mass spectrometry [J].

Hoja, H ;

Marquet, P ;

Verneuil, B ;

Lotfi, H ;

Dupuy, JL ;

Lachatre, G .

JOURNAL OF CHROMATOGRAPHY B, 1997, 692 (02) :329-335

[4] QUANTIFICATION OF LSD AND N-DEMETHYL-LSD IN URINE BY GAS-CHROMATOGRAPHY RESONANCE ELECTRON-CAPTURE IONIZATION MASS-SPECTROMETRY [J].

LIM, HK ;

ANDRENYAK, D ;

FRANCOM, P ;

FOLTZ, RL ;

JONES, RT .

ANALYTICAL CHEMISTRY, 1988, 60 (14) :1420-1425

[5] CHROMATOGRAPHIC AND MASS-SPECTROMETRIC METHODS FOR DETERMINATION OF LYSERGIC-ACID DIETHYLAMIDE (LSD) AND METABOLITES IN BODY-FLUIDS [J].

NELSON, CC ;

FOLTZ, RL .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 580 (1-2) :97-109

[6] GAS-CHROMATOGRAPHIC ELECTRON-IMPACT MASS FRAGMENTOMETRIC DETERMINATION OF LYSERGIC-ACID DIETHYLAMIDE IN URINE [J].

PAUL, BD ;

MITCHELL, JM ;

BURBAGE, R ;

MOY, M ;

SROKA, R .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 529 (01) :103-112

[7] Recent advances in chromatographic and mass spectrometric methods for determination of LSD and its metabolites in physiological specimens [J].

Reuschel, SA ;

Eades, D ;

Foltz, RL .

JOURNAL OF CHROMATOGRAPHY B, 1999, 733 (1-2) :145-159

[8] Detection of lysergic acid diethylamide (LSD) in urine by gas chromatography-ion trap tandem mass spectrometry [J].

Sklerov, JH ;

Kalasinsky, KS ;

Ehorn, CA .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1999, 23 (06) :474-478

[9] ANALYSIS OF LSD IN HUMAN-BODY FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, FLUORESCENCE SPECTROSCOPY AND RADIOIMMUNOASSAY [J].

TWITCHETT, PJ ;

FLETCHER, SM ;

SULLIVAN, AT ;

MOFFAT, AC .

JOURNAL OF CHROMATOGRAPHY, 1978, 150 (01) :73-84

[10]

Yarnold BM, 1999, PSYCHOL REP, V85, P431

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