Microsatellite and CAPS markers for ethylene-related genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes, and their variation in melon (Cucumis melo L.)

被引：9

作者：

Akashi, Y ^{[1
]}

Shiomi, S ^{[1
]}

Kubo, Y ^{[1
]}

Masuda, M ^{[1
]}

Kato, K ^{[1
]}

机构：

[1] Okayama Univ, Fac Agr, Okayama 7008530, Japan

来源：

BREEDING SCIENCE | 2001年 / 51卷 / 02期

关键词：

Cucumis melo; ACC oxidase; ACC synthase; CAPS; microsatellite; phylogeny;

D O I：

10.1270/jsbbs.51.107

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, Me-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)(n) and (A)(n) were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

引用

页码：107 / 112

页数：6

共 32 条

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