An efficient micropropagation system for Tylophora indica:: an endangered, medicinally important plant

被引:48
作者
Faisal, Mohd. [1 ]
Ahmad, Naseem [1 ]
Anis, Mohammad [1 ]
机构
[1] Aligarh Muslim Univ, Dept Bot, Plant Biotechnol Lab, Aligarh 202002, Uttar Pradesh, India
关键词
asclepiadaceae; conservation; medicinal plant; micropropagation; Tylophora indica;
D O I
10.1007/s11816-007-0025-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要
An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Tylophora indica (Burm. f.) Merrill, via enhanced axillary bud proliferation from nodal explants collected from young shoots of a two-year-old plant. The physiological effects of growth regulators [6-benzyladenine (BA), kinetin (Kin) thidiazuron (TDZ), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or a-naphthalene acetic acid (NAA)], ascorbic acid (AA), different strengths of Murashige and Skoog ( MS) medium and various pH levels on in vitro morphogenesis were investigated. The highest number (8.6 +/- 0.71) of shoots and the maximum average shoot length (5.2 +/- 0.31 cm) were recorded on MS medium supplemented with 2.5 mu M BA, 0.5 mu M NAA and 100 mg/l AA at pH 5.8. Rooting was best achieved on half-strength MS medium augmented with 0.5 mu M IBA. The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing garden soil and grown in a greenhouse with a 90% survival rate. The regenerated plants did not show any immediate detectable phenotypic variation. The described method can be successfully employed for large-scale multiplication and long-term in vitro conservation of T. indica.
引用
收藏
页码:155 / 161
页数:7
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