Enhancement of Bone Marrow-Derived Mesenchymal Stem Cell Osteogenesis and New Bone Formation in Rats by Obtusilactone A

被引：8

作者：

Lin, Yi-Hsiung ^{[1
,2
,3
]}

Chen, Chung-Yi ^{[4
]}

Chou, Liang-Yin ^{[1
,2
,3
]}

Chen, Chung-Hwan ^{[1
,5
,6
,7
]}

Kang, Lin ^{[8
]}

Wang, Chau-Zen ^{[1
,2
,3
,9
]}

机构：

[1] Kaohsiung Med Univ, Orthopaed Res Ctr, Kaohsiung 807, Taiwan

[2] Kaohsiung Med Univ, Dept Physiol, Coll Med, Kaohsiung 807, Taiwan

[3] Kaohsiung Med Univ, Grad Inst Med, Coll Med, Kaohsiung 807, Taiwan

[4] Fooyin Univ, Sch Med & Hlth Sci, Kaohsiung 807, Taiwan

[5] Kaohsiung Med Univ, Dept Orthopaed, Coll Med, Kaohsiung 807, Taiwan

[6] Kaohsiung Med Univ, Kaohsiung Municipal Ta Tung Hosp, Dept Orthoped, Kaohsiung 807, Taiwan

[7] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Div Adult Reconstruct Surg, Dept Orthoped, Kaohsiung 807, Taiwan

[8] Natl Cheng Kung Univ, Natl Cheng Kung Univ Hosp, Dept Obstet & Gynecol, Coll Med, Tainan 701, Taiwan

[9] Kaohsiung Med Univ Hosp, Dept Med Res, Kaohsiung 807, Taiwan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 11期

关键词：

bone marrow derived mesenchymal stem cells (BMSCs); osteogenesis; bone formation; Cinnamomum kotoense; obtusilactone A; CINNAMOMUM-KOTOENSE; SIGNALING PATHWAY; STROMAL CELLS; TGF-BETA; DIFFERENTIATION; THERAPY; ALLELES; LEAVES; BMP2;

D O I：

10.3390/ijms18112422

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias. Micro-CT images and H&E staining showed more trabecular bone at the needle-implanted site in the OA group than the normal saline group. Thus, OA confers an osteoinductive effect on BMSCs via induction of osteogenic marker gene expression, such as BMP2 and Runx2 expression and subsequently elevates ALP activity and mineralization, followed by enhanced trabecular bone formation in rat tibias. Therefore, OA is a potential osteoinductive drug to stimulate new bone formation by BMSCs.

引用

页数：13

共 22 条

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