Comparison between immunofluorescence and immunomagnetic techniques of cytometry

被引：21

作者：

Tchikov, V ^{[1
]}

Schütze, S ^{[1
]}

Krönke, MK ^{[1
]}

机构：

[1] Univ Kiel, Inst Immunol, D-24105 Kiel, Germany

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 1999年 / 194卷 / 1-3期

关键词：

cytometry; magnetophoresis; cell; antibody; antigen; immunomicrospheres; nanoparticles;

D O I：

10.1016/S0304-8853(98)00567-8

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Magnetophoresis and fluorescence activated cell sorting were used for evaluation of immunochemical properties of magnetic particles and fluorescent probes. The HLA-Bw6 antigen on surfaces of REH cells was detected with a primary monoclonal antibody and a secondary antibody coupled with fluorescent molecules or magnetic particles. Magnetophoresis can find applications in biology and medicine for measuring percentages of cell subpopulations. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：242 / 247

页数：6