Abnormal anal cytology in high-risk human papilloma virus infection in HIV-infected Australians

Objective: To assess the prevalence of abnormal anal cytology and high-risk human papilloma virus ( HPV)-type infection in HIV-infected people with a CD4 cell count >300 cells/mu l. Methods: The clinic-based patient population included 126 HIV-infected people: 124 men who have sex with men, and two women ( median age 45 years; CD4 cell count >300 cells/mu l). Anal cytology swabs were placed into liquid-based medium for HPV typing by Hybrid Capture-2 assay and cytological assessment, by a single cytopathologist. Results: 106 ( 84%) participants were infected with high-risk HPV; 17 ( 14%) had no high-risk types of HPV detected; three ( 2%) had no HPV assay result because of an inadequate sample. Sixteen ( 13%) participants had cytological evidence of high-grade squamous intraepithelial ( HGSIL) changes, 100% of whom had high-risk HPV types detected, and 13 ( 10%) had atypical squamous cells of undetermined significance with possible high-grade changes ( ASCUS-H), 92% of whom had high-risk HPV types detected. Low-grade changes ( LSIL) were detected in 24 ( 19%) participants, 96% of whom had high-risk HPV types, 32 ( 25%) had ASCUS with 88% high-risk HPV types, 30 ( 24%) had normal cytology with 73% high-risk HPV types, and 11 ( 9%) samples were inadequate for cytological assessment. The odds ratio of participants with high-risk HPV having abnormal anal cytology on anal swab was 5.03 ( 95% CI 1.45 to 17.39). Discussion: High-risk HPV types are common in this HIV+ population with a CD4 cell count >300 cells/mu l. The presence of high-risk HPV types was associated with abnormal anal cytology such as HGSIL and ASCUS-H.