A nuclear localization signal in the matrix of spleen necrosis virus (SNV) does not allow efficient gene transfer into quiescent cells with SNV-derived vectors

被引：8

作者：

Caron, MC ^{[1
]}

Caruso, M ^{[1
]}

机构：

[1] Univ Laval, Ctr Hosp Univ Quebec, Lhotel Dieu Quebec, Ctr Rech Cancerol, Quebec City, PQ G1R 2J6, Canada

来源：

VIROLOGY | 2005年 / 338卷 / 02期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

retrovirus; spleen necrosis virus; quiescent cells; vector; gene therapy; RD114;

D O I：

10.1016/j.virol.2005.05.024

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A major limitation in gene therapy for vectors derived from Moloney murine leukemia virus (MLV) is that they only deliver genes into dividing cells. In this study, a careful comparison of spleen necrosis virus (SNV)-derived vectors with MLV and human immunodeficiency virus (HIV)-1 retroviral vectors indicated that SNV vectors (,an deliver genes 4-fold more efficiently than MLV vectors into aphidicolin-arrested cells, although at a 25-fold lower efficiency than HIV-1-derived vectors. Furthermore, the addition of a NLS in the SNV matrix (MA) that mimics the one located in HIV-1 MA did not increase the ability of SNV vectors to transfer genes into arrested cells. Also, we found that the RD114 envelope was able to pseudotype SNV viral particles in a very efficient manner. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：292 / 296

页数：5

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