Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein

被引：26

作者：

Oldham, NJ

Krieger, J

Breer, H

Svatos, A

机构：

[1] Max Planck Inst Chem Ecol, D-07745 Jena, Germany

[2] Univ Stuttgart, Inst Physiol, D-70593 Stuttgart, Germany

[3] Acad Sci Czech Republic, Inst Organ Chem & Biochem, CZ-16610 Prague, Czech Republic

来源：

CHEMICAL SENSES | 2001年 / 26卷 / 05期

关键词：

D O I：

10.1093/chemse/26.5.529

中图分类号：

B84 [心理学]; C [社会科学总论]; Q98 [人类学];

学科分类号：

03 ; 0303 ; 030303 ; 04 ; 0402 ;

摘要：

Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an Escherichia coli expression system, has been found to contain (11Z)-octadecenoic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidation procedure is described to overcome what would appear to be a general problem with recombinant lepidopteran PBPs.

引用

页码：529 / 531

页数：3

共 14 条

[1] PURIFICATION AND CHARACTERIZATION OF AN ODORANT-BINDING PROTEIN FROM COW NASAL TISSUE
BIGNETTI, E
CAVAGGIONI, A
PELOSI, P
PERSAUD, KC
SORBI, RT
TIRINDELLI, R
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 149 (02): : 227 - 231
[2] BLAU K, 1993, HDB DERIVATES CHROMA, P11
[3] PROTEIN-STRUCTURE ENCODES THE LIGAND-BINDING SPECIFICITY IN PHEROMONE BINDING-PROTEINS
DU, GH
PRESTWICH, GD
[J]. BIOCHEMISTRY, 1995, 34 (27) : 8726 - 8732
[4] Expression, purification, and crystal structure determination of recombinant human epidermal-type fatty acid binding protein
Hohoff, C
Börchers, T
Rüstow, B
Spener, F
van Tilbeurgh, H
[J]. BIOCHEMISTRY, 1999, 38 (38) : 12229 - 12239
[5] Olfactory reception in invertebrates
Krieger, J
Breer, H
[J]. SCIENCE, 1999, 286 (5440) : 720 - 723
[6] Oldham NJ, 2000, ANGEW CHEM INT EDIT, V39, P4341, DOI 10.1002/1521-3773(20001201)39:23<4341::AID-ANIE4341>3.0.CO
[7] 2-1
[8] Oldham NJ, 1999, RAPID COMMUN MASS SP, V13, P331, DOI 10.1002/(SICI)1097-0231(19990315)13:5<331::AID-RCM487>3.3.CO
[9] 2-1
[10] Discrimination of pheromone enantiomers by two pheromone binding proteins from the gypsy moth Lymantria dispar
Plettner, E
Lazar, J
Prestwich, EG
Prestwich, GD
[J]. BIOCHEMISTRY, 2000, 39 (30) : 8953 - 8962

← 1 2 →