Coordinated Regulation of ATF2 by miR-26b in γ-Irradiated Lung Cancer Cells

被引：36

作者：

Arora, Himanshu ^{[1
]}

Qureshi, Rehana ^{[1
]}

Park, Ae-Kyung ^{[1
]}

Park, Woong-Yang ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Med, Dept Biomed Sci Biochem & Mol Biol, Seoul, South Korea

来源：

PLOS ONE | 2011年 / 6卷 / 08期

关键词：

RESPONSE ELEMENT; TARGET GENES; MICRORNA; EXPRESSION; BINDING; IDENTIFICATION; INVOLVEMENT; ACTIVATION; RESISTANCE; PATHWAYS;

D O I：

10.1371/journal.pone.0023802

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

MicroRNA regulates cellular responses to ionizing radiation (IR) through translational control of target genes. We analyzed time-series changes in microRNA expression following gamma-irradiation in H1299 lung cancer cells using microarray analysis. Significantly changed IR-responsive microRNAs were selected based on analysis of variance analysis, and predicted target mRNAs were enriched in mitogen-activated protein kinase (MAPK) signaling. Concurrent analysis of time-series mRNA and microRNA profiles uncovered that expression of miR-26b was down regulated, and its target activating transcription factor 2 (ATF2) mRNA was up regulated in gamma-irradiated H1299 cells. IR in miR-26b overexpressed H1299 cells could not induce expression of ATF2. When gamma-Jun N-terminal kinase activity was inhibited using SP600125, expression of miR-26b was induced following gamma-irradiation in H1299 cells. From these results, we concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway.

引用

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