Vaccination of B-CLL patients with autologous dendritic cells can change the frequency of leukemia antigen-specific CD8+ T cells as well as CD4+CD25+FoxP3+ regulatory T cells toward an antileukemia response

被引：74

作者：

Hus, I. ^{[2
]}

Schmitt, M. ^{[1
]}

Tabarkiewicz, J. ^{[3
]}

Radej, S. ^{[3
]}

Wojas, K. ^{[1
,3
]}

Bojarska-Junak, A. ^{[3
]}

Schmitt, A. ^{[1
]}

Giannopoulos, K. ^{[3
]}

Dmoszynska, A. ^{[2
]}

Rolinski, J. ^{[3
]}

机构：

[1] Univ Ulm, Dept Internal Med 3, D-89081 Ulm, Germany

[2] Med Univ Lublin, Dept Hematooncol, Lublin, Poland

[3] Med Univ Lublin, Dept Clin Immunol, Lublin, Poland

来源：

LEUKEMIA | 2008年 / 22卷 / 05期

关键词：

chronic lymphocytic leukemia; immunotherapy; RHAMM/CD168; dendritic cells; tumor cell lysates; magnetic cell separation;

D O I：

10.1038/leu.2008.29

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Recently, we described that vaccination with allogeneic dendritic cells (DCs) pulsed with tumor cell lysate generated specific CD8 + T cell response in patients with B-cell chronic lymphocytic leukemia (B-CLL). In the present study, the potential of autologous DCs pulsed ex vivo with tumor cell lysates to stimulate antitumor immunity in patients with B-CLL in early stages was evaluated. Twelve patients at clinical stage 0-2 as per Rai were vaccinated intradermally up to eight times with a mean number of 7.4 x 10(6) DCs pulsed with B-CLL cell lysate. We observed a decrease of peripheral blood leukocytes and CD19 +/CD5 + leukemic cells in five patients, three patients showed a stable disease and four patients progressed despite DC vaccination. A significant increase of specific cytotoxic CD8 + T lymphocytes against the leukemia-associated antigens RHAMM or fibromodulin was detected in four patients after DC vaccination. In patients with a clinical response, an increase of interleukin 12 (IL-12) serum levels and a decrease of the frequency of CD4 + CD25 + FOXP3 + T regulatory cells were observed. Taken together, the study demonstrated that vaccination with autologous DC in CLL patients is feasible and safe. Immunological and to some extend hematological responses could be noted, justifying further investigation on this immunotherapeutical approach.

引用

页码：1007 / 1017

页数：11

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Chang AE, 2002, CLIN CANCER RES, V8, P1021

[3]

Generation of dendritic cells from positively selected CD14+ monocytes for anti-tumor immunotherapy [J].