Burst production of superoxide anion in human endothelial cells by lysophosphatidylcholine

被引：49

作者：

Kugiyama, K ^{[1
]}

Sugiyama, S ^{[1
]}

Ogata, N ^{[1
]}

Oka, H ^{[1
]}

Doi, H ^{[1
]}

Ota, Y ^{[1
]}

Yasue, H ^{[1
]}

机构：

[1] Kumamoto Univ, Sch Med, Div Cardiol, Kumamoto 8608556, Japan

来源：

ATHEROSCLEROSIS | 1999年 / 143卷 / 01期

关键词：

free radicals; endothelium; lipids;

D O I：

10.1016/S0021-9150(98)00288-3

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study examined whether lysophosphatidylcholine (lysoPC), an atherogenic lipid, may stimulate production of O-2(.-), in cultured human endothelial cells. Production of O-2(.-) was detected by bis-N-methylacridinium nitrate (lucigenin)-elicited chemiluminescence. LysoPC was found to induce burst production of O-2(.-), peaked at 2 similar to 4 min after the stimulation, in intact endothelial cells. LysoPC also stimulated NADH-dependent production of O-2(.-) in particulate fraction of the cells, and the action of lysoPC was inhibited by diphenyliodonium. The results suggested that lysoPC stimulated production of O-2(.-) partly through membrane-associated NADH-dependent O-2(.-) production systems. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：201 / 204

页数：4

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