Purification and properties of a basic endo-1,3-beta-glucanase from rice (Oryza sativa L)

被引：17

作者：

Akiyama, T ^{[1
]}

Kaku, H ^{[1
]}

Shibuya, N ^{[1
]}

机构：

[1] NATL INST AGROBIOL RESOURCES,DEPT CELL BIOL,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT AND CELL PHYSIOLOGY | 1996年 / 37卷 / 05期

关键词：

amino acid sequence; 1,3-beta-glucanase (EC 3.2.1.39); rice (Oryza saliva);

D O I：

10.1093/oxfordjournals.pcp.a029002

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A 1,3-beta-glucanase purified from rice grain is a 33 kDa monomer with a pI of greater than or equal to 10.4. The enzyme was determined to be an endo-1,3-beta-glucanase (EC 3.2.1.39) by end product analysis using Laminaria digitata laminarin as substrate. Its amino-terminal amino acid sequence revealed strong homology to an endo-1,3-beta-glucanase from barley.

引用

页码：702 / 705

页数：4

共 23 条

[1] STUDIES ON BETA-GLUCANASES .5. PARTIAL-PURIFICATION AND PROPERTIES OF AN ENDO-1,3-BETA-D-GLUCANASE FROM GERMINATED RYE
BALLANCE, GM
MANNERS, DJ
[J]. PHYTOCHEMISTRY, 1978, 17 (09) : 1539 - 1543
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] MOLECULAR CHARACTERIZATION OF A PEA BETA-1,3-GLUCANASE INDUCED BY FUSARIUM-SOLANI AND CHITOSAN CHALLENGE
CHANG, MM
HADWIGER, LA
HOROVITZ, D
[J]. PLANT MOLECULAR BIOLOGY, 1992, 20 (04) : 609 - 618
[4] PURIFICATION, CHARACTERIZATION, AND ACTION-PATTERN STUDIES ON ENDO-(1-] 3)-BETA-D-GLUCANASE FROM RHIZOPUS-ARRHIZUS QM 1032
CLARK, DR
JOHNSON, J
CHUNG, KH
KIRKWOOD, S
[J]. CARBOHYDRATE RESEARCH, 1978, 61 (MAR) : 457 - 477
[5] CDNA CLONING AND CHARACTERIZATION OF A PUTATIVE 1,3-BETA-D-GLUCANASE TRANSCRIPT INDUCED BY FUNGAL ELICITOR IN BEAN CELL-SUSPENSION CULTURES
EDINGTON, BV
LAMB, CJ
DIXON, RA
[J]. PLANT MOLECULAR BIOLOGY, 1991, 16 (01) : 81 - 94
[6] MOLECULAR-CLONING, SEQUENCE-ANALYSIS AND EXPRESSION OF THE GENE FOR CATALASE-PEROXIDASE (CPEA) FROM THE PHOTOSYNTHETIC BACTERIUM RHODOBACTER-CAPSULATUS B10
FORKL, H
VANDEKERCKHOVE, J
DREWS, G
TADROS, MH
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 214 (01): : 251 - 258
[7] HOY PB, 1995, PLANT J, V7, P367
[8] HOY PB, 1989, PLANT MOL BIOL, V13, P31
[9] PURIFICATION AND PROPERTIES OF 3 (1-]3)-BETA-D-GLUCANASE ISOENZYMES FROM YOUNG LEAVES OF BARLEY (HORDEUM-VULGARE)
HRMOVA, M
FINCHER, GB
[J]. BIOCHEMICAL JOURNAL, 1993, 289 : 453 - 461
[10] BIOLOGICAL FUNCTION OF PATHOGENESIS-RELATED PROTEINS - 4 PR PROTEINS OF TOBACCO HAVE 1,3-BETA-GLUCANASE ACTIVITY
KAUFFMANN, S
LEGRAND, M
GEOFFROY, P
FRITIG, B
[J]. EMBO JOURNAL, 1987, 6 (11) : 3209 - 3212

← 1 2 3 →