Optimization extraction, preliminary characterization and antioxidant activity in vitro of polysaccharides from Stachys sieboldii Miq. tubers

被引：36

作者：

Feng, Kai ^{[1
]}

Chen, Wei ^{[1
]}

Sun, Liwei ^{[1
]}

Liu, Jianzeng ^{[1
]}

Zhao, Yangxin ^{[1
]}

Li, Luxi ^{[1
]}

Wang, Yuxing ^{[1
]}

Zhang, Wenjing ^{[1
]}

机构：

[1] Beihua Univ, Tradit Chinese Med Biotechnol Innovat Ctr Jilin P, Fengman 132013, Jilin, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2015年 / 125卷

关键词：

Polysaccharides; Response surface methodology; Stachys sieboldi Miq; Characterization; Antioxidant; CRUDE POLYSACCHARIDES; ANTITUMOR; ANTIBACTERIAL; MYCELIA; LEAF; OIL;

D O I：

10.1016/j.carbpol.2015.02.026

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Response surface methodology was used to optimize the extraction conditions of water-soluble polysaccharides from Stachys sieboldii Miq. tubers. A central composite design was used to optimize the extraction processing parameters. The optimum extraction conditions are as follows: extraction temperature, 95 degrees C; extraction time, 2.5 h; water to raw material ratio, 16; and extraction frequency, 3. Under the optimized conditions, an experimental yield of 9.21 +/- 0.18%, which is in good agreement with the predicted yield, was obtained. Purified polysaccharide SSP 11-a was successfully obtained using diethylaminoethanol-Sepharose and Sepharose CL-6B column chromatography. SSP 11-a was found to be an acidic polysaccharide fraction with an average molecular weight of 168 kDa and composed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose. In vitro antioxidant activity assays suggested that SSP II-a presents high scavenging activity toward superoxide anion, hydroxyl, and 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radicals but relatively lower scavenging activity toward 1,1-diphenyl-2-picrylhydrazyl radicals. The results indicated that response surface methodology is an effective method for the extraction of polysaccharides from S. sieboldii Miq. tubers and the polysaccharides could be explored as a potential antioxidant agent for use in medicine or functional food. (C)2015 Elsevier Ltd. All rights reserved.

引用

页码：45 / 52

页数：8

共 34 条

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