Comparison of Transepidermal Water Loss and Laser Scanning Microscopy Measurements to Assess Their Value in the Characterization of Cutaneous Barrier Defects

被引：26

作者：

Czaika, V. ^{[1
,2
]}

Alborova, A. ^{[3
]}

Richter, H. ^{[3
]}

Sterry, W. ^{[3
]}

Vergou, T. ^{[5
]}

Antoniou, C. ^{[5
]}

Lademann, J. ^{[3
]}

Koch, S. ^{[1
,2
,4
]}

机构：

[1] Ostbrandenburg Tumorzentrum, Helios Klinikum Bad Saarow, Dept Gen Med, Internal Clin 1, DE-15526 Bad Saarow Pieskow, Germany

[2] Ostbrandenburg Tumorzentrum, Inst Pathol, DE-15526 Bad Saarow Pieskow, Germany

[3] Charite, Ctr Expt & Appl Cutaneous Physiol CCP, Dept Dermatol & Allergol, D-13353 Berlin, Germany

[4] Ernst Moritz Arndt Univ Greifswald, Inst Hyg, Greifswald, Germany

[5] Univ Athens, Hosp A Syggros, Athens, Greece

来源：

SKIN PHARMACOLOGY AND PHYSIOLOGY | 2012年 / 25卷 / 01期

关键词：

In vivo laser scanning microscopy; Transepidermal water loss; Wound healing; Suction blister technique; SKIN BARRIER; STRATUM-CORNEUM; THICKNESS;

D O I：

10.1159/000330486

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100227 [皮肤病学];

摘要：

The exact qualitative and quantitative analysis of wound healing processes is a decisive prerequisite for optimizing wound care and for therapy control. Transepidermal water loss (TEWL) measurements are considered to be the standard procedure for assessing the progress of epidermal wound healing. The damage to the stratum corneum correlates with an increased loss of water through the skin barrier. This method is highly susceptible to failure by environmental factors, in particular by temperature and moisture. This study was aimed at comparing TEWL measurements and in vivo laser scanning microscopy (LSM) for the characterization of the epidermal wound healing process. LSM is a high-resolution in vivo method permitting to analyze the kinetics and dynamics of wound healing at a cellular level. While the TEWL values for the individual volunteers showed a wide scattering, LSM permitted the wound healing process to be clearly characterized at the cellular level. However, a comparison between the two methods was very difficult, because the results provided by LSM were images and not numerical. Therefore, a scoring system was set up which evaluates the stages of wound healing. Thus, the healing process could be numerically described. This method is independent of any environmental factors. Providing morphologically qualitative and numerically quantitative analyses of the wound healing process and being far less vulnerable to failure, LSM is advantageous over TEWL. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：39 / 46

页数：8

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