Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology

被引:91
作者
Millar, AA
Wrischer, M
Kunst, L [1 ]
机构
[1] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada
[2] Rudjer Boskovic Inst, Mol Genet Unit, Zagreb 10000, Croatia
关键词
D O I
10.1105/tpc.10.11.1889
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transgenic Arabidopsis plants overexpressing the Arabidopsis FATTY ACID ELONGATION1 gene under the control of the 35S promoter from cauliflower mosaic virus accumulated very-long-chain fatty acids (VLCFAs) throughout the plant. In some transformants, C20 and C22 VLCFAs accounted for >30% of the total fatty acids, accumulating at the expense of C16 and C18 fatty acids. These C20 and C22 fatty acids were incorporated into all of the major membrane glycerolipid classes. Plants with a high VLCFA content displayed a dramatically altered morphology, which included the failure of flowering shoots to elongate, a modified spatial pattern of siliques, an altered floral phenotype, and a large accumulation of anthocyanins. In addition, these plants also exhibited a unique alteration of the chloroplast membrane structure. We discuss a possible role for VLCFAs in establishing the shape/curvature of the membranes, which in turn may affect the shape of the cell and ultimately that of the whole plant.
引用
收藏
页码:1889 / 1902
页数:14
相关论文
共 34 条
[1]   RICINOLEIC ACID BIOSYNTHESIS AND TRIACYLGLYCEROL ASSEMBLY IN MICROSOMAL PREPARATIONS FROM DEVELOPING CASTOR-BEAN (RICINUS-COMMUNIS) ENDOSPERM [J].
BAFOR, M ;
SMITH, MA ;
JONSSON, L ;
STOBART, K ;
STYMNE, S .
BIOCHEMICAL JOURNAL, 1991, 280 :507-514
[2]   BIOSYNTHESIS OF VERNOLEATE (CIS-12-EPOXYOCTADECA-CIS-9-ENOATE) IN MICROSOMAL PREPARATIONS FROM DEVELOPING ENDOSPERM OF EUPHORBIA-LAGASCAE [J].
BAFOR, M ;
SMITH, MA ;
JONSSON, L ;
STOBART, K ;
STYMNE, S .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 303 (01) :145-151
[3]   Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean [J].
Broun, P ;
Somerville, C .
PLANT PHYSIOLOGY, 1997, 113 (03) :933-942
[4]   FLUXES THROUGH THE PROKARYOTIC AND EUKARYOTIC PATHWAYS OF LIPID-SYNTHESIS IN THE 16-3 PLANT ARABIDOPSIS-THALIANA [J].
BROWSE, J ;
WARWICK, N ;
SOMERVILLE, CR ;
SLACK, CR .
BIOCHEMICAL JOURNAL, 1986, 235 (01) :25-31
[5]   FATTY-ACID COMPOSITION OF LEAF LIPIDS DETERMINED AFTER COMBINED DIGESTION AND FATTY-ACID METHYL-ESTER FORMATION FROM FRESH TISSUE [J].
BROWSE, J ;
MCCOURT, PJ ;
SOMERVILLE, CR .
ANALYTICAL BIOCHEMISTRY, 1986, 152 (01) :141-145
[6]   JASMONIC ACID METHYL JASMONATE ACCUMULATE IN WOUNDED SOYBEAN HYPOCOTYLS AND MODULATE WOUND GENE-EXPRESSION [J].
CREELMAN, RA ;
TIERNEY, ML ;
MULLET, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4938-4941
[7]   CELLULOSE BIOSYNTHESIS [J].
DELMER, DP ;
AMOR, Y .
PLANT CELL, 1995, 7 (07) :987-1000
[8]  
DENKAMP JAFO, 1985, TRENDS BIOCHEM SCI, V10, P320
[9]  
Eccleston VS, 1996, PLANTA, V198, P46, DOI 10.1007/BF00197585
[10]   ACYL-COA ELONGASE FROM A HIGHER-PLANT (LUNARIA-ANNUA) - METABOLIC INTERMEDIATES OF VERY-LONG-CHAIN ACYL-COA PRODUCTS AND SUBSTRATE-SPECIFICITY [J].
FEHLING, E ;
MUKHERJEE, KD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1082 (03) :239-246