A stereoselective fluorogenic assay for aldolases:: Detection of an anti-selective aldolase catalytic antibody

被引：37

作者：

Jourdain, N ^{[1
]}

Carlón, RP ^{[1
]}

Reymond, JL ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

TETRAHEDRON LETTERS | 1998年 / 39卷 / 51期

关键词：

D O I：

10.1016/S0040-4039(98)02139-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Stereoisomeric aldols 6a-d undergo retroaldolization to give 3-oxopropyl umbelliferyl ether 5 and subsequently umbelliferone 4 by beta-elimination, leading to a fluorescence increase at lambda(em) = 460 +/- 20 nm (lambda(ex) = 360 +/- 20 nm). This fluorogenic assay for aldolases operates in cell culture media and can be used to search for new stereoselective aldolase biocatalysts. Aldolase antibody 38C2 (Aldrich no. 47,995-0) catalyzes stereoselectively the retroaldolization of(S)-anti aldol 6c. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：9415 / 9418

页数：4

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