Two-dimensional planar chromatography of tropane alkaloids from Datura innoxia Mill.

被引：18

作者：

Gadzikowska, M ^{[1
]}

Petruczynik, A ^{[1
]}

Waksmundzka-Hajnos, M ^{[1
]}

Hawryl, M ^{[1
]}

Jozwiak, G ^{[1
]}

机构：

[1] Med Univ, Fac Pharm, Dept Inorgan Chem, PL-20081 Lublin, Poland

来源：

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2005年 / 18卷 / 102期

关键词：

2D-TLC; tropane alkaloids; Datura innoxia Mill; selectivity; identification; quantitation;

D O I：

10.1556/JPC.18.2005.2.7

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Plant extracts from Datura innoxia Mill. contain tropane alkaloids which are pharmacologically active, and compounds used in medicine are isolated industrially from the plant. Control of the composition and content of these alkaloids is, therefore, an important task. Thin-layer chromatography is an effective method for analysis of these compounds, because they are difficult to detection with UV light. In the work discussed in this paper separation of closely related alkaloids was achieved by two-dimensional TLC in optimized NP and RP systems by use of multiphase plates. The work enabled identification of tropane alkaloids present in plant extracts. Normal-phase separation on silica layers was optimized for quantitative analysis of the alkaloids scopolamine and tropine and the sum of atropine and homatropine.

引用

页码：127 / 131

页数：5

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