Functional analysis for peripheral myelin protein PASII/PMP22: Is it a member of claudin superfamily?

被引:9
作者
Takeda, Y
Notsu, T
Kitamura, K
Uyemura, K
机构
[1] Tokyo Metropolitan Inst Gerontol, Dept Cell Recognit, Itabashi Ku, Tokyo 1730015, Japan
[2] Keio Univ, Sch Med, Dept Physiol, Shinjuku Ku, Tokyo 1600016, Japan
[3] Saitama Med Sch, Dept Physiol, Moroyama, Saitama 3500495, Japan
关键词
PASII/PMP22; peripheral myelin; claudin superfamily; glycoprotein; differentiation;
D O I
10.1023/A:1010927001378
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two major glycoproteins, P0 and PASII/PMP22. are specifically expressed in peripheral myelin. Point mutations of these proteins and over or under expression of PASII/PMP22 cause various hereditary peripheral neuropathies. P0 is well characterized as a major adhesion molecule in PNS myelin, but the function of PASII/PMP22 is still unknown. Recently, an oligodendrocyte-specific protein (OSP) was identified as a member of the claudin family and as a component of tight junctions of central myelins. Since PASII/PMP22 shows similarity in structure to OSP, which is a tetraspan membrane protein, we speculated if PASII/PMP22 could be a member of claudin superfamily. The primary structure of PASII/PMP22 showed a significant homology of 48% and a 21% identity with the OSP sequence. Exogenous expression of PASII/PMP22 in C6 cells significantly inhibited BrdU incorporation to the cells. The C6 cells stably transfected with PASII/PMP22 cDNA showed no homophilic cell adhesive activity. When dorsal root ganglion (DRG) neurons were cocultured on PASII/PMP22 expressing cells, both neurite extension and branching of DRG neurons were significantly inhibited. These results indicate that PASII/PMP22 may play a role in a turning point of Schwann cell development from proliferation to differentiation. On the other hand, the cells expressing claudin family proteins are reported to show strong cell adhesive activity and an ability to form tight junctions with neighboring cells. For this reason, we currently do not have any functional data supporting that PASII/PMP22 is the member of claudin superfamily.
引用
收藏
页码:599 / 607
页数:9
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