Infection with Langat flavivirus or expression of the envelope protein induces apoptotic cell death

被引:41
作者
Prikhod'ko, GG
Prikhod'ko, EA
Cohen, JI
Pletnev, AG
机构
[1] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
[2] NIAID, Clin Invest Lab, NIH, Bethesda, MD 20892 USA
关键词
flaviviruses; Langat virus and envelope glycoprotein E; apoptosis; DEVDase activity; Neuro-2a and Vero cells; caspases and inhibitors;
D O I
10.1006/viro.2001.0980
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Langat (LGT) flavivirus, derived from infectious full-length cDNA clone 636, was investigated for its apoptotic activities in mouse neuroblastoma (Neuro-2a) and simian kidney (Vero and LLC-MK2) cells. The hallmark of apoptosis, cleavage of cellular DNA, was observed 48 h after infection of Vero, LLC-MK2, and Neuro-2a cells by electrophoresis analysis. Apoptosis in infected cells was also confirmed by TUNEL assay. LGT-infected Neuro-2a cells showed an increase in caspase-3-like protease (DEVDase) activity. Expression of the major envelope glycoprotein (E) alone reduced cell viability in both Vero and Neuro-2a cells, and the baculovirus P35 protein, which inhibits multiple caspases, completely blocked this effect Cleavage of cellular DNA was observed in E gene-transfected Vero cells by TUNEL assay, Expression of E protein or caspase-9 resulted in activation of caspase-3-like proteases in Neuro-2a cells. The caspase-3-like protease specific inhibitor, Ac-DEVD-CHO peptide, partially inhibited E protein- or caspase-9-induced apoptosis in Neuro-2a cells. These observations indicate that infection of cells with LGT virus or expression of LGT virus E protein induces apoptosis through a caspase-3-like protease pathway.
引用
收藏
页码:328 / 335
页数:8
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