Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources

被引:171
作者
Gouda, MK [1 ]
Swellam, AE [1 ]
Omar, SH [1 ]
机构
[1] Univ Alexandria, Fac Sci, Dept Bot, Alexandria, Egypt
关键词
PHB; Bacillus megaterium; cane molasses; corn steep liquor;
D O I
10.1078/0944-5013-00104
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Poly(hydroxybutyric acid) (PHB) and other biodegradable polyesters are promising candidates for the development of environment-friendly, totally biodegradable plastics. The use of cane molasses and corn steep liquor, two of the cheapest substrates available in Egypt, may help to reduce the cost of producing such biopolyesters. In this work, the effect of different carbon sources was studied. Maximum production of PHB was obtained with cane molasses and glucose as sole carbon sources (40.8, 39.9 per mg cell dry matter, respectively). The best growth was obtained with 3% molasses, while maximum yield of PHB (46.2% per mg cell dry matter) was obtained with 2% molasses. Corn steep liquor was the best nitrogen source for PHB synthesis (32.7 mg per cell dry matter), on the other hand, best growth was observed when ammonium chloride, ammonium sulphate, ammonium oxalate or ammonium phosphate were used as nitrogen sources.
引用
收藏
页码:201 / 207
页数:7
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