Simultaneous liquid chromatographic determination of lamotrigine, oxcarbazepine monohydroxy derivative and felbamate in plasma of patients with epilepsy

被引:64
作者
Contin, M [1 ]
Balboni, M [1 ]
Callegati, E [1 ]
Candela, C [1 ]
Albani, F [1 ]
Riva, R [1 ]
Baruzzi, A [1 ]
机构
[1] Univ Bologna, Lab Clin Neuropharmacol, Neurol Clin, Dept Neurol Sci, I-40123 Bologna, Italy
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 828卷 / 1-2期
关键词
lamotrigine; oxcarbazepine main active metabolite; felbamate; HPLC analysis; therapeutic drug monitoring;
D O I
10.1016/j.jchromb.2005.09.009
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A very simple and fast method has been developed and validated for simultaneous determination of the new generation antiepileptic drugs (AEDs) lamotrigine (LTG), oxcarbazepine's (OXC) main active metabolite monohydroxycarbamazepine and felbamate in plasma of patients with epilepsy using high-performance liquid chromatography (HPLC) with spectrophotometric detection. Plasma sample (500 mu L) pre-treatment was based on simple deproteinization by acetonitrile. Liquid chromatographic analysis was carried out on a Synergi 4 mu m Hydro-RP, 150 mm x 4 mm I.D. column, using a mixture of potassium dihydrogen phosphate buffer (50 mM, pH 4.5) and acetonitrile/methanol (3/1) (65:35, v/v) as the mobile phase, at a flow rate of 1.0 mL/min. The UV detector was set at 210 nm. Calibration curves were linear (mean correlation coefficient > 0.999 for all the three analytes) over a range of 1-20 mu g/mL for lamotrigine, 2-40 mu g/mL for monohydroxycarbamazepine and 10-120 mu g/mL for felbamate. Both intra and interassay precision and accuracy were lower than 7.5% for all three analytes. Absolute recoveries ranged between 100 and 104%. The present procedure describes for the first time the simultaneous determination of these three new antiepileptic drugs. The simple sample pre-treatment, combined with the fast chromatographic run permit rapid processing of a large series of patient samples. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:113 / 117
页数:5
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