Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein

被引：45

作者：

Cowley, SC ^{[1
]}

Av-Gay, Y ^{[1
]}

机构：

[1] Univ British Columbia, Dept Med, Div Infect Dis, Vancouver, BC V5Z 3J5, Canada

来源：

GENE | 2001年 / 264卷 / 02期

关键词：

green fluorescent proteins; transcriptional fusion; translational fusion;

D O I：

10.1016/S0378-1119(01)00336-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two green fluorescent protein (Gfp) fusion Vectors were constructed for use in Mycobacterium spp. The first plasmid facilitates quantification of mycobacterial promoter activity. The second vector permits construction of translational fusions of mycobacterial proteins to Gfp in order to study subcellular localization including protein secretion. Using this translational fusion construct, we verify that a Gfp fusion to the putative secreted M. tuberculosis protein ChoD is translocated to the extracellular milieu when cloned and expressed in Mycobacterium smegniatis. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：225 / 231

页数：7

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