Cyclosporine-induced renal artery smooth muscle contraction is associated with increases in the phosphorylation of specific contractile regulatory proteins

被引:18
作者
Beall, A
Epstein, A
Woodrum, D
Brophy, CM
机构
[1] Med Coll Georgia, Dept Surg, Augusta, GA 30912 USA
[2] Med Coll Georgia, Dept Med, Inst Mol Med & Genet, Augusta, GA 30912 USA
[3] Med Coll Georgia, Dept Cell Biol & Anat, Augusta, GA 30912 USA
[4] Augusta Vet Adm Med Ctr, Augusta, GA 30901 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1999年 / 1449卷 / 01期
关键词
cyclosporine; smooth muscle; vascular; myosin light chain; heat shock protein;
D O I
10.1016/S0167-4889(98)00169-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cyclosporine A (CSA) is a type 2B phosphatase inhibitor which can induce contraction of renal artery smooth muscle. In this investigation, we examined the phosphorylation events associated with CSA-induced contraction of bovine renal artery smooth muscle. Contractile responses were determined in a muscle bath and the corresponding phosphorylation events were determined with whole cell phosphorylation and two-dimensional gel electrophoresis. CSA-induced contractions were associated with increases in the phosphorylation of the 20 kDa myosin Light chains (MLC20) and different isoforms of the small heat shock protein, HSP27. Cyclic nucleotide-dependent relaxation of CSA-induced contractions was associated with increases in the phosphorylation of another small heat shock protein, HSP20, and decreases in the phosphorylation of the MLC20, and some isoforms of HSP27. These data suggest that CSA-induced contraction and relaxation of vascular smooth muscle is associated with increases in the phosphorylation of specific contractile regulatory proteins. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:41 / 49
页数:9
相关论文
共 43 条
[1]   PROTEIN-PHOSPHORYLATION DURING THE CONTRACTION RELAXATION CONTRACTION CYCLE OF ARTERIAL SMOOTH-MUSCLE [J].
BARANY, M ;
POLYAK, E ;
BARANY, K .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 294 (02) :571-578
[2]  
Beall AC, 1997, J BIOL CHEM, V272, P11283
[3]  
BENNDORF R, 1994, J BIOL CHEM, V269, P20780
[4]   HSP27 IS A MEDIATOR OF SUSTAINED SMOOTH-MUSCLE CONTRACTION IN RESPONSE TO BOMBESIN [J].
BITAR, KN ;
KAMINSKI, MS ;
HAILAT, N ;
CEASE, KB ;
STRAHLER, JR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (03) :1192-1200
[5]   Cellular mechanisms of cyclic nucleotide-induced vasorelaxation [J].
Brophy, CM ;
Whitney, EG ;
Lamb, S ;
Beall, A .
JOURNAL OF VASCULAR SURGERY, 1997, 25 (02) :390-397
[6]   Thrombin activates MAPKAP2 kinase in vascular smooth muscle [J].
Brophy, CM ;
Woodrum, D ;
Dickinson, M ;
Beall, A .
JOURNAL OF VASCULAR SURGERY, 1998, 27 (05) :963-969
[7]   CYCLOSPORINE-INDUCED ACUTE RENAL DYSFUNCTION IN THE RAT - EVIDENCE OF ARTERIOLAR VASOCONSTRICTION WITH PRESERVATION OF TUBULAR FUNCTION [J].
ENGLISH, J ;
EVAN, A ;
HOUGHTON, DC ;
BENNETT, WM .
TRANSPLANTATION, 1987, 44 (01) :135-141
[8]  
EPSTEIN A, 1998, SURGERY, V27, P963
[9]  
GAESTEL M, 1992, J BIOL CHEM, V267, P21607
[10]  
GAR MD, 1990, AM J PHYSIOL, V258, pF211