Identification of trinucleotide repeat ligands with a FRET melting assay

被引：16

作者：

Amrane, Samir ^{[1
]}

De Cian, Anne ^{[1
]}

Rosu, Frederic ^{[2
]}

Kaiser, Markus ^{[3
]}

De Pauw, Edwin ^{[2
]}

Teulade-Fichou, Marie-Paule ^{[3
]}

Mergny, Jean-Louis ^{[1
]}

机构：

[1] Museum Natl Hist Nat, CNRS, INSERM, USM 503,U565, F-75005 Paris, France

[2] Univ Liege, Mass Spectrometry Lab, Chem Inst B6c, B-4000 Liege, Belgium

[3] Inst Curie, CNRS, Sect Rech, UMR 176, F-91405 Orsay, France

来源：

CHEMBIOCHEM | 2008年 / 9卷 / 08期

关键词：

DNA ligands; DNA recognition; DNA; FRET; nucleic acids;

D O I：

10.1002/cbic.200800062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligonds that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that mocrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.

引用

页码：1229 / 1234

页数：6

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