Archaeal communities in two disparate deteriorated ancient wall paintings:: detection, identification and temporal monitoring by denaturing gradient gel electrophoresis

被引：54

作者：

Piñar, G

Saiz-Jimenez, C

Schabereiter-Gurtner, C

Blanco-Varela, MT

Lubitz, W

Rölleke, S

机构：

[1] Univ Vienna, Inst Microbiol & Genet, A-1030 Vienna, Austria

[2] CSIC, Inst Recursos Nat & Agrobiol, E-41080 Seville, Spain

[3] CSIC, Inst Eduardo Torroja Ciencias Construcc, Madrid 28033, Spain

[4] Genalysis GmbH, D-149430 Luckenwalde, Germany

来源：

FEMS MICROBIOLOGY ECOLOGY | 2001年 / 37卷 / 01期

关键词：

wall painting; 16S rDNA; denaturing gradient gel electrophoresis; clone library; Archaea;

D O I：

10.1016/S0168-6496(01)00144-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the present study we describe the detection of Archaea in two different deteriorated ancient wall paintings, located in Austria and Spain, under different humidity/salinity regimes and climates. Archaeal communities were analyzed by combination of denaturing gradient gel electrophoresis (DGGE) of PCR-amplified DNA encoding 16S rRNA and the construction of clone libraries. DGGE analysis was used for temporal monitoring of the archaeal communities as well as for screening of the clone libraries. Selected clones were compared with band patterns obtained from the original samples and sequenced. Sequence analyses documented the existence of species belonging to well-characterized halophilic and alkaliphilic Archaea. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：45 / 54

页数：10

共 46 条

[1] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].