Scanning electrochemical microscopy imaging of DNA microarrays using methylene blue as a redox-active intercalator

被引：50

作者：

Wain, Andrew J. ^{[1
]}

Zhou, Feimeng ^{[1
]}

机构：

[1] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

来源：

LANGMUIR | 2008年 / 24卷 / 09期

关键词：

D O I：

10.1021/la703922v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Scanning electrochemical microscopy (SECM) has been employed in the imaging of DNA microarrays fabricated on gold substrates using methylene blue (MB) as a redox-active intercalator and ferrocyanide as the SECM mediator in solution. MB intercalated between base pairs of immobilized ds-DNA is electrochemically reduced via electron transfer from the underlying gold substrate, and the product is reoxidized in solution by SECM tip-generated ferricyanide. The resulting feedback current allows a heterogeneous electron-transfer rate constant for the MB-intercalated DNA to be deduced. Moreover, DNA microarray spots can be imaged at a detection level of 14 fmol/spot for ds-DNA consisting of 15 base pairs. Microarrays prepared using 20 mu M DNA solutions are easily visualized, and the feasibility of detecting base pair mismatches is also demonstrated.

引用

页码：5155 / 5160

页数：6

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