The central pyrogenic action of interleukin-6 is related to nuclear translocation of STAT3 in the anteroventral preoptic area of the rat brain

被引：12

作者：

Hübschle, T ^{[1
]}

Harré, EM ^{[1
]}

Meyerhof, W ^{[1
]}

Pehl, U ^{[1
]}

Roth, J ^{[1
]}

Gerstberger, R ^{[1
]}

机构：

[1] Univ Giessen, Inst Vet Physiol, D-35392 Giessen, Germany

来源：

JOURNAL OF THERMAL BIOLOGY | 2001年 / 26卷 / 4-5期

关键词：

fever; ventromedial preoptic area (VMPO); vascular organ of the lamina terminalis (OVLT); cytokines; signal transducer and activator of transcription (STAT3); immunohistochemistry;

D O I：

10.1016/S0306-4565(01)00034-1

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

(1) In animals treated with i.c.v. pyrogen free saline Constitutive STAT3-expression was low and predominantly detected within the cytoplasm of large cells in the medio-caudal hypothalamus. (2) Wistar rats treated with i.c.v. IL-6 (100 200 ng) developed a clear febrile response 90-120 min post treatment and showed a strong STAT3-upregulation and -translocation into cell nuclei at various fore- and hindbrain sites that was already observed 15-30 min after the treatment. (3) The most intense IL-6-induced STAT3-activation pattern was found within the vascular organ of the lamina terminalis and the adjacent ventromedial preoptic area. (4) STAT3-immunohistochemistry therefore represents a novel tool to functionally map IL-6 actions on the brain. The results further support the importance of the anteroventral preoptic area as main central IL-6 target involved in the mediation of fever responses. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：299 / 305

页数：7

共 23 条

[1]

IL-6-regulated transcription factors [J].

Akira, S .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1997, 29 (12) :1401-1418

[2]

BLATTEIS CM, 1992, PROG BRAIN RES, V91, P409

[3]

Differential Fos expression induced by IL-1 beta and IL-6 in rat hypothalamus and pituitary gland [J].

Callahan, TA ;

Piekut, DT .

JOURNAL OF NEUROIMMUNOLOGY, 1997, 73 (1-2) :207-211

[4]

Interleukin (IL)-6 gene expression in the central nervous system is necessary for fever response to lipolysaccharide or IL-1 beta: A study on IL-6-deficient mice [J].

Chai, Z ;

Gatti, S ;

Toniatti, C ;

Poli, V ;

Bartfai, T .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (01) :311-316

[5]

Mechanisms of CNS response to systemic immune challenge: the febrile response [J].

Elmquist, JK ;

Scammell, TE ;

Saper, CB .

TRENDS IN NEUROSCIENCES, 1997, 20 (12) :565-570

[6]

Elmquist JK, 1996, J COMP NEUROL, V371, P85, DOI 10.1002/(SICI)1096-9861(19960715)371:1<85::AID-CNE5>3.0.CO

[7]

2-H

[8]

Elmquist JK, 1996, J COMP NEUROL, V374, P315, DOI 10.1002/(SICI)1096-9861(19961021)374:3<315::AID-CNE1>3.0.CO

[9]

2-4

[10]

Leptin-induced nuclear translocation of STAT3 immunoreactivity in hypothalamic nuclei involved in body weight regulation [J].

Hübschle, T ;

Thom, E ;

Watson, A ;

Roth, J ;

Klaus, S ;

Meyerhof, W .

JOURNAL OF NEUROSCIENCE, 2001, 21 (07) :2413-2424

← 1 2 3 →