Kinetics of human immunodeficiency virus type 1 (HIV) DNA integration in acutely infected cells as determined using a novel assay for detection of integrated HIV DNA

被引：87

作者：

Vandegraaff, N

Kumar, R

Burrell, CJ

Li, P

机构：

[1] Natl Ctr HIV Virol Res, Inst Med & Vet Sci, Infect Dis Labs, Adelaide, SA 5000, Australia

[2] Univ Adelaide, Dept Mol Biosci, Adelaide, SA, Australia

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 22期

关键词：

D O I：

10.1128/JVI.75.22.11253-11260.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have developed a novel linker-primer PCR assay for the detection and quantification of integrated human immunodeficiency virus type 1 (HIV) DNA. This assay reproducibly allowed the detection of 10 copies of integrated HIV DNA, in a background of 2 x 10(5) cell equivalents of human chromosomal DNA, without amplifying extrachromosomal HIV DNA. We have used this assay and a near-synchronous one-step T-cell infection model to investigate the kinetics of viral DNA accumulation following HIV infection. We report here that integrated HIV DNA started accumulating 1 h after the first appearance of extrachromosomal viral DNA and accounted for similar to 10% of the total HIV DNA synthesized in the first round of viral replication. These results highlight the efficient nature of integrase-mediated HIV integration in infected T cells.

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页码：11253 / 11260

页数：8

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