Determination and pharmacokinetics of ergometrine maleate in rabbit blood with on line microdialysis sampling and fluorescence detection

The study describes a flow injection on-line microdialysis system for in vivo monitoring of ergometrine maleate in rabbit blood with fluorescence detection. A flow-through microdialysis probe was used for intravenous sampling by pumping of the blood from the tested rabbit through the flow-through microdialysis probe located outside the living system at a flow rate of 15 mu l min(-1). The perfusion rate is 5 mu l min(-1). The ergometrine maleate in the dialysate was detected on-line with a flow injection fluorescence system after the ergometrine maleate administration (0.2 mg kg(-1), i.v.). The dialysate sample volume was about 15 mu l. The system was linearly related to the concentration of ergometrine maleate in the range 1-140 ng ml(-1) (r=0.9989) with a detection limit 0.3 ng ml(-1) (3 sigma). The pharmacokinetic parameters of ergometrine maleate were calculated utilizing the pharmacokinetic software 'NDST-21' by a one-compartmental open model. (c) 2004 Elsevier B.V. All rights reserved.