TLC analysis of a corrinoid compound from dark muscle of the yellowfin tuna (Thunnus albacares)

被引:10
作者
Nishioka, Michiko
Tanioka, Yuri
Miyamoto, Emi
Enotnoto, Toshiki
Watanabe, Futnio [1 ]
机构
[1] Tottori Univ, Fac Agr, Sch Agr Biol & Environm Sci, Tottori 6808553, Japan
[2] Kochi Womens Univ, Dept Hlth Sci, Kochi, Japan
[3] Osaka Prefecture Univ, Grad Sch Agr & Biol Sci, Sakai, Osaka 591, Japan
[4] Nagasaki Int Univ, Dept Hlth & Nutr, Sasebo, Japan
[5] Ishikawa Prefectural Univ, Dept Food Sci, Ishikawa, Japan
关键词
yellowfin tuna; Thunnus albacares; dark muscle; HPLC; TLC; vitamin b12;
D O I
10.1080/10826070701451605
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A significant amount of vitamin B 12 (52.9 +/- 8.9 mu g/ 100 g) was found in the dark muscle of the yellowfin tuna (Thunnus albacares), in comparison to that of the light muscle. A corrinoid compound was purified to homogeneity from the dark muscle and partially characterized. TLC and HPLC patterns of the purified corrinoid compound were identical to those of authentic vitamin B12. These results indicate that dark muscle of the yellowfin tuna would be an excellent source of vitamin 1312 for humans and aminals.
引用
收藏
页码:2245 / 2252
页数:8
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