Effect of calcium phosphate addition to alginate microspheres: Modulation of enzyme release kinetics and improvement of cell adhesion

被引：7

作者：

Barrias, CC

Ribeiro, CC

Rodrigues, D

Miranda, MCS

Barbosa, MA

机构：

[1] INEB, Inst Biomed Engn, Lab Biomat, P-4150180 Oporto, Portugal

[2] Univ Porto, Fac Engn Porto, Dept Fis, Oporto, Portugal

[3] Inst Super Engn Porto, Dept Fis, Oporto, Portugal

来源：

BIOCERAMICS 17 | 2005年 / 284-286卷

关键词：

alginate; calcium titanium phosphate; cell adhesion; enzyme release; hydroxyapatite; microspheres;

D O I：

10.4028/www.scientific.net/KEM.284-286.689

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

0805 [材料科学与工程]; 080502 [材料学];

摘要：

In this study, the addition of calcium phosphate powders to an alginate matrix was evaluated as a strategy to modulate enzyme release-kinetics from alginate microspheres and, simultaneously, to improve cell adhesion to the polymer. Pre-adsorption of the enzyme to the ceramic powders resulted in a more adequate release pattern. The ratio of ceramic-to-polymer had a pronounced effect on osteoblast adhesion to microspheres. Cells were only able to spread on microspheres with the highest percentage of ceramic (0.4 w/w using a 1.5% w/v alginate solution).

引用

页码：689 / 692

页数：4

共 3 条

[1]

Increasing hydroxyapatite incorporation into poly(methylmethacrylate) cement increases osteoblast adhesion and response [J].