Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodinium cohnii

被引：105

作者：

de la Jara, A

Mendoza, H ^{[1
]}

Martel, A

Molina, C

Nordströn, L

de la Rosa, V

Díaz, R

机构：

[1] Inst Tecnol Canarias, Dept Biotecnol, Sta Lucia 35119, Spain

[2] Univ Las Palmas Gran Canaria, Ctr Algol Aplicada, Telde 35214, Spain

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2003年 / 15卷 / 05期

关键词：

Crypthecodinium cohnii; DHA; flow cytometry; lipid content; neutral lipids; nile red; polar lipids; PUFA;

D O I：

10.1023/A:1026007902078

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Since flow cytometry allows rapid, simultaneous and quantitative measurements related to cell morphology and physiologicy, the lipid-specific fluorescent dye, Nile Red, was employed for the in vivo lipid quantification of Crypthecodinium cohnii, a heterotrophic marine dinoflagellate rich in polyunsaturated long chain fatty acids. The fluorescence signal was linearly correlated with the neutral and polar lipid content as determined by gravimetric techniques. A significant correlation of NR signal was also observed between the polar to neutral lipid ratio and docohexaenoic acid per cell. The results demonstrate a method for rapid lipid quantification that can be used in the selection, isolation and culture control of C cohnii clones with high lipid and DHA content.

引用

页码：433 / 438

页数：6

共 20 条

[1] Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red [J].