Human placental cytotrophoblasts attract monocytes and CD56bright natural killer cells via the actions of monocyte inflammatory protein 1α

被引:125
作者
Drake, PM
Gunn, MD
Charo, IF
Tsou, CL
Zhou, Y
Huang, L
Fisher, SJ
机构
[1] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA
[5] Univ Calif San Francisco, Program Biol Sci, San Francisco, CA 94143 USA
[6] Univ Calif San Francisco, Dept Stomatol, San Francisco, CA 94143 USA
[7] Univ Calif San Francisco, Gladstone Inst Cardiovasc Dis, San Francisco, CA 94143 USA
[8] Univ Calif San Francisco, Inst Cardiovasc Res, San Francisco, CA 94143 USA
[9] Univ Calif San Francisco, Daiichi Res Ctr, San Francisco, CA 94143 USA
[10] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
[11] Duke Univ, Dept Med, Div Cardiol, Durham, NC 27710 USA
关键词
chemokine; pregnancy; leukocyte; immune tolerance; trophoblast;
D O I
10.1084/jem.193.10.1199
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
During human pregnancy, the specialized epithelial cells of the placenta (cytotrophoblasts) come into direct contact with immune cells in several locations. In the fetal compartment of the placenta, cytotrophoblast stem cells lie adjacent to macrophages (Hofbauer cells) that reside within the chorionic villus stroma, At sites of placental attachment to the mother, invasive cytotrophoblasts encounter specialized maternal natural killer (NK) cells (CD56bright), macrophages, and T cells that accumulate within the uterine wall during pregnancy. Hare we tested the hypothesis that fetal cytotrophoblasts carl direct the migration of these maternal immune cells. First, we assayed the chemotactic activity of cytotrophoblast conditioned medium samples, using human peripheral blood mononuclear cells as targets. The placental samples preferentially attracted NK cells (both CD56(dim) and CD56(bright)), monocytes. and T cells, suggesting that our hypothesis was correct. A screen to idrntifv chemokine activity through the induction of a Ca transfected with individual chemokine receptors suggested that cytotrophoblasts secreted monocyte inflammatory protein (MIP)-1 alpha. This was confirmed by localizing the corresponding mRNA and protein, both in vitro and in vivo. MIP-1 alpha protein in conditioned medium was further characterized by immunoblotting and enzyme-linked immunosorbent assay. Immunodepletion of MIP-1 alpha from cytotrophoblast conditioned medium showed that this chemokine was responsible for a significant portion of the induced monocyte and CD56(bright) NK cell chemotaxis. These data suggest the specific conclusion that cytotrophoblasts carl attract monocytes and CD56(bright) NK cells by producing MIP-1 alpha and the more general hypothesis that these cells may organize and act on leukocytes at the maternal-fetal interface.
引用
收藏
页码:1199 / 1212
页数:14
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