Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae)

被引：29

作者：

Mesquita, N

Cunha, C

Hänfling, B

Carvalho, GR

Zé-Zé, L

Tenreiro, R

Coelho, MM

机构：

[1] Fac Ciencias Lisboa, Dept Biol Anim, Ctr Biol Ambiental, P-1749016 Lisbon, Portugal

[2] Univ Hull, Dept Biol Sci, Mol Ecol & Fisheries Genet Lab, Kingston Upon Hull HU6 7RX, N Humberside, England

[3] Fac Ciencias Lisboa, Dept Biol Vegetal, Ctr Genet & Biol Mol, P-1749016 Lisbon, Portugal

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 04期

关键词：

Cyprinidae; di- and tetra-nucleotide repeats; microsatellites; primers; Squalius aradensis;

D O I：

10.1046/j.1471-8286.2003.00515.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe the isolation and characterization of six polymorphic microsatellite loci for Squalius aradensis. The number of observed alleles per locus ranged from three to 16 and the observed heterozygosity from 0.22 to 0.95. These primers will be useful in determining the population structure of S. aradensis and for conservation genetics of this endangered and endemic species. Furthermore, successful cross-species amplification in S. alburnoides and Chondrostoma lusitanicum suggests that a wider amplification of these markers is possible.

引用

页码：572 / 574

页数：3

共 10 条

[1] ISOLATION OF HUMAN SIMPLE REPEAT LOCI BY HYBRIDIZATION SELECTION [J].