Human immunodeficiency virus type 1 N-terminal capsid mutants that exhibit aberrant core morphology and are blocked in initiation of reverse transcription in infected cells
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作者:
Tang, SX
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机构:NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
Tang, SX
Murakami, T
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机构:NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
Murakami, T
Agresta, BE
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机构:NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
Agresta, BE
Campbell, S
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机构:NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
Campbell, S
Freed, EO
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机构:NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
Freed, EO
Levin, JG
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机构:NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
Levin, JG
机构:
[1] NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA
[2] NIAID, Mol Microbiol Lab, Bethesda, MD 20892 USA
[3] NCI, HIV Drug Resistance Program, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
A group of conserved hydrophobic residues faces the interior of the coiled-coil-like structure within the N-terminal domain of the human immunodeficiency virus type 1 (HIV-1) capsid protein (CA). It has been suggested that these residues are important for maintaining stable structure and functional activity. To investigate this possibility, we constructed two HIV-1 clones, in which Trp23 or Phe40 was changed to Ala. We also constructed a third mutant, D51A, which has a mutation that destroys a salt bridge between Pro1 and Asp51. All three mutants are replication defective but produce virus particles. Mutant virions contain all of the viral proteins, although the amount and stability of CA are decreased and levels of virion-associated integrase are reduced. The mutations do not affect endogenous reverse transcriptase activity; however, the mutants are blocked in their ability to initiate reverse transcription in infected cells and no minus-strand strong-stop DNA is detected. The defect in reverse transcription is associated with striking defects in the morphology of mutant virus cores, as determined by transmission electron microscopy. Our data indicate that the mutations made in this study disrupt CA structure and prevent proper maturation of virus cores. We propose that this results in a defect in core stability or in an early postentry event preceding reverse transcription.
机构:
Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Bowzard, JB
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Wills, JW
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Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Wills, JW
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Craven, RC
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Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
机构:
Penn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Cairns, TM
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Craven, RC
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Penn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USA
机构:
Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Bowzard, JB
;
Wills, JW
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机构:
Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Wills, JW
;
Craven, RC
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机构:
Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
机构:
Penn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USA
Cairns, TM
;
Craven, RC
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h-index: 0
机构:
Penn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USAPenn State Univ, Coll Med, Milton S Hershey Med Ctr, Dept Microbiol & Immunol, Hershey, PA 17033 USA